APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 , 293T
Predict Reactivity
Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888319
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cofilin 1 , CFL , HEL-S-15 , cofilin
Cellular Localization
Nucleus matrix , Cytoplasm , cytoskeleton
Background
The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]
Database
Research Area
DATA IMAGES
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GTX632582 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000.
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GTX632582 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX632582 ICC/IF Image
Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cell membrane by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Cofilin 1 stained by Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX632582 IHC-P Image
Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX632582 IP Image
Immunoprecipitation of Cofilin 1 protein from 293T whole cell extracts using 5 μg of Cofilin 1 antibody [GT217] (GTX632582). Western blot analysis was performed using Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:500. EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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GTX632582 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX632582 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Cofilin 1 antibody [GT217] (GTX632582). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cofilin 1 antibody [GT217] (GTX632582). Be the first to share your experience with this product.