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Cofilin 1 antibody [GT217]

Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582
Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582
Anti-Cofilin 1 antibody [GT217] used in Immunoprecipitation (IP). GTX632582
Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582
Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582
Anti-Cofilin 1 antibody [GT217] used in IHC (Paraffin sections) (IHC-P). GTX632582

Cat No. GTX632582

Host

Mouse

Clonality

Monoclonal

Clone Name

GT217

Isotype

IgG2b

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat-2 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888319

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cofilin 1 , CFL , HEL-S-15 , cofilin

Cellular Localization

Nucleus matrix , Cytoplasm , cytoskeleton

Background

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582

GTX632582 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000.

Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582

GTX632582 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody [GT217] used in Immunoprecipitation (IP). GTX632582

GTX632582 IP Image

Immunoprecipitation of Cofilin 1 protein from 293T whole cell extracts using 5 μg of Cofilin 1 antibody [GT217] (GTX632582).
Western blot analysis was performed using Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582

GTX632582 WB Image

Cofilin 1 antibody [GT217] detects Cofilin 1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000.

Anti-Cofilin 1 antibody [GT217] used in Western Blot (WB). GTX632582

GTX632582 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody [GT217] used in IHC (Paraffin sections) (IHC-P). GTX632582

GTX632582 IHC-P Image

Cofilin 1 antibody [GT217] detects Cofilin 1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Cofilin 1 antibody [GT217] (GTX632582) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for Cofilin 1 antibody [GT217] (GTX632582). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Cofilin 1 antibody [GT217] (GTX632582). Be the first to share your experience with this product.
SDS
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