APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2
Predict Reactivity
Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888056
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cofilin 1 , CFL , HEL-S-15 , cofilin
Cellular Localization
Nucleus matrix , Cytoplasm , cytoskeleton
Background
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/templates/default/js/lightbox/images/loading.gif "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
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GTX628804 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628804 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628804 ICC/IF Image
Cofilin 1 antibody [GT567] detects Cofilin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: Cofilin 1 protein stained by Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX628804 IHC-P Image
Cofilin 1 antibody [GT567] detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Cofilin 1 antibody [GT567] (GTX628804) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628804 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX628804 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
There are currently no references for Cofilin 1 antibody [GT567] (GTX628804). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cofilin 1 antibody [GT567] (GTX628804). Be the first to share your experience with this product.
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20161013_WB_shRNA_watermark_18121411_273.jpg "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20170615_WB_M_18121411_312.jpg "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20150807_IFA_18121411_986.jpg "Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_IHC_18121411_745.jpg "Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20170615_WB_R_18121411_292.jpg "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/small/GTX628804/GTX628804_41204_20230210_WB_TPM_watermark_23021401_497.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20161013_WB_shRNA_watermark_18121411_273.jpg "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20170615_WB_M_18121411_312.jpg "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20150807_IFA_18121411_986.jpg "Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_IHC_18121411_745.jpg "Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20170615_WB_R_18121411_292.jpg "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")
![Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804](/upload/website/prouct_img/normal/GTX628804/GTX628804_41204_20230210_WB_TPM_watermark_23021401_497.webp "Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804")