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Cofilin 1 antibody [GT567]

Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804
Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804
Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804
Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804
Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804
Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804

Cat No. GTX628804

Host Mouse
Clonality Monoclonal
Clone Name GT567
Isotype IgG2a
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cofilin 1,Cfl,Hel-S-15,Cofilin,Cfl1

Cellular Localization

Nucleus matrix , Cytoplasm , cytoskeleton

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804

GTX628804 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804

GTX628804 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody [GT567] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628804

GTX628804 ICC/IF Image

Cofilin 1 antibody [GT567] detects Cofilin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: Cofilin 1 protein stained by Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804

GTX628804 WB Image

Cofilin 1 antibody [GT567] detects CFL1 protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
12% SDS-PAGE
Cofilin 1 antibody [GT567] (GTX628804) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody [GT567] used in IHC (Paraffin sections) (IHC-P). GTX628804

GTX628804 IHC-P Image

Cofilin 1 antibody [GT567] detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Cofilin 1 antibody [GT567] (GTX628804) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cofilin 1 antibody [GT567] used in Western Blot (WB). GTX628804

GTX628804 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (GTX628804) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169