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Cofilin 1 antibody

KO/KD Orthogonal
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102156
Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156
Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156

Cat. No. GTX102156

Reference 19
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Cofilin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949971

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cofilin 1 , CFL , HEL-S-15 , cofilin

Cellular Localization

Nucleus matrix , Cytoplasm , cytoskeleton

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102156

GTX102156 IHC-P Image

Cofilin 1 antibody detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Cofilin 1 antibody (GTX102156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156

GTX102156 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cofilin 1 (non-muscle)(GTX102156) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156

GTX102156 ICC/IF Image

Cofilin 1 antibody detects Cofilin 1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 0.012 N NaOH/PBS for 1 min (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cofilin 1 protein stained by Cofilin 1 antibody (GTX102156) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE

Application Reference

Lee SH et al. Int J Mol Sci 2020; 21 (16) Parkin Coordinates Platelet Stress Response in Diabetes Mellitus: A Big Role in a Small Cell.
Chen SY et al. Stem Cells Transl Med 2020; 9 (4) : 499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187 : 113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Williams HC et al. Lab Invest 2019; 99 (3) : 399-410 The cofilin phosphatase slingshot homolog 1 restrains angiotensin II-induced vascular hypertrophy and fibrosis in vivo.
Diego Lucero et al. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2019; 1865 (2) : 158537 Interleukin 10 promotes macrophage uptake of HDL and LDL by stimulating fluid-phase endocytosis.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

Cofilin 1 antibody Cat. No. GTX102156

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Stem Cells Transl Med in 2020.PMID: 31943851
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Lee SH et al. Int J Mol Sci 2020; 21 (16) Parkin Coordinates Platelet Stress Response in Diabetes Mellitus: A Big Role in a Small Cell.
Chen SY et al. Stem Cells Transl Med 2020; 9 (4):499-517 Exosomal 2',3'-CNP from mesenchymal stem cells promotes hippocampus CA1 neurogenesis/neuritogenesis and contributes to rescue of cognition/learning deficiencies of damaged brain.
Application : WB
Reactivity : Mouse
Chen JF et al. J Pharm Biomed Anal 2020; 187:113142 Proteomic analysis of Antrodia Cinnamomea-induced ER stress in liver cancer cells.
Application : WB
Reactivity : Human
Williams HC et al. Lab Invest 2019; 99 (3):399-410 The cofilin phosphatase slingshot homolog 1 restrains angiotensin II-induced vascular hypertrophy and fibrosis in vivo.
Bulletin of the Korean Chemical Society 2019; (Epub) Transgelin Depletion is Critical for the TGFβ1?mediated Initiation of PLCγ1?Cofilin?driven Morphological and Migratory Changes in MDA?MB?231 Cells.
Diego Lucero et al. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2019; 1865 (2):158537 Interleukin 10 promotes macrophage uptake of HDL and LDL by stimulating fluid-phase endocytosis.
Application : WB
Reactivity : Mouse
Yu HI et al. J Pharm Biomed Anal 2018; 160:344-350 Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells.
Application : WB
Reactivity : Human
Chou HC et al. Life Sci 2018; (Epub) Proteomic analysis of honokiol-induced cytotoxicity in thyroid cancer cells.
Application : WB
Reactivity : Human
Chang SJ et al. Arch Biochem Biophys 2018; 647:10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Dettling S et al. Clin Cancer Res 2018; (Epub) Identification of CRKII, CFL1, CNTN1, NME2, and TKT as Novel and Frequent T-cell Targets in Human IDH-Mutant Glioma.
Application : IHC-Fr
Reactivity : Human
Stakaityt? G et al. J Virol 2017; (Epub) Merkel cell polyomavirus small T antigen drives cell motility via Rho-GTPase-induced filopodia formation.
Application : WB
Reactivity : Human
Yang HH et al. Proteomics Clin Appl 2016; (Epub) the putative involvement of actin binding proteins and cytoskeleton proteins in pathological mechanisms of * cystitis - revealed by a prospective pilot study using proteomic approaches.
Application : WB
Reactivity : Human
Wang CY et al. CLINICS ONCOL 2016; 1:1015 Over-Expression of Cofilin-1 Suppressed Mobility of Lung Cancer Cells is Associated with Down-Regulation of SNAIL-1 and Induction of Let-7.
Application : WB
Reactivity : Human
MarceloF.Montenegro et al. FreeRadicalBiologyandMedicine 2015; 85:288-94 Nox4-dependent activation of cofilin mediates VSMC reorientation in response to cyclic stretching
Application : WB
Reactivity : Human
Tsai CH et al. Biochim Biophys Acta 2015; 1852 (5):851-61 Over-expression of cofilin-1 suppressed growth and invasion of cancer cells is associated with up-regulation of let-7 microRNA.
Application : WB
Reactivity : Human
Toyo-Oka K et al. J Neurosci 2014; 34 (36):12168-81 14-3-3ε and ζ Regulate Neurogenesis and Differentiation of Neuronal Progenitor Cells in the Developing Brain.
Application : WB
Reactivity : Mouse
Wu CL et al. Electrophoresis 2013; 34 (3):388-96 Hyaluronic acid-dependent protection against alkali-burned human corneal cells.
Application : ICC/IF
Reactivity : Human
Lin LT et al. Mol Imaging Biol 2013; 15 (3):262-72 A comparative study of primary and recurrent human glioblastoma multiforme using the small animal imaging and molecular expressive profiles.
Application : WB
Reactivity : Human
Maheswaranathan M et al. J Biol Chem 2011; 286 (41):35430-7 Platelet-derived growth factor (PDGF) regulates Slingshot phosphatase activity via Nox1-dependent auto-dephosphorylation of serine 834 in vascular smooth muscle cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169