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Cofilin 1 antibody

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156
Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156
Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156

Cat No. GTX102156

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

293T , A431 , NIH-3T3 , PC-12 , Rat2

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

cofilin 1 , CFL , HEL-S-15 , cofilin

Cellular Localization

Nucleus matrix , Cytoplasm , cytoskeleton

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody (GTX102156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
12% SDS PAGE
GTX102156 diluted at 1:10000

Anti-Cofilin 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102156

GTX102156 IHC-P Image

Cofilin 1 antibody detects CFL1 protein at cytosol on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Cofilin 1 antibody (GTX102156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Cofilin 1 antibody detects CFL1 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat-2 whole cell lysate/extract
12% SDS-PAGE
Cofilin 1 antibody (GTX102156) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Western Blot (WB). GTX102156

GTX102156 WB Image

Sample (30 µg of whole cell lysate)
A:NIH-3T3
12% SDS PAGE
GTX102156 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156

GTX102156 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Cofilin 1 (non-muscle)(GTX102156) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Cofilin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102156

GTX102156 ICC/IF Image

Cofilin 1 antibody detects Cofilin 1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 0.012 N NaOH/PBS for 1 min (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cofilin 1 protein stained by Cofilin 1 antibody (GTX102156) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

REFERENCE
REVIEW

Cofilin 1 antibody Cat No. GTX102156

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 25-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 20 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169