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Cofilin 1 (phospho Ser3) antibody

Anti-Cofilin 1 (phospho Ser3) antibody used in IHC (Paraffin sections) (IHC-P). GTX12866
Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX12866

Cat No. GTX12866

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
50 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% Glycerol, 150mM NaCl, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human cofilin around the phosphorylation site of serine 3 (M-A-Sp-G-V).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_369396

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CFL,CFL1,HELS15,cofilin,cofilin 1,Cofilin 1

Cellular Localization

Nucleus matrix,Cytoplasm,Cytoskeleton,Cell projection,Ruffle membrane,Lamellipodium membrane

Background

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Anti-Cofilin 1 (phospho Ser3) antibody used in IHC (Paraffin sections) (IHC-P). GTX12866

GTX12866 IHC-P Image

IHC-P image using GTX12866 - Detection of Cofilin by IHC-P in human breast carcinoma tissue.

Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX12866

GTX12866 WB Image

Western blot analysis of extracts from COLO205 cells using cofilin antibody (Lane 1 and 2) and cofilin (phospho-Ser3) antibody (GTX12866, Lane 3 and 4)

REFERENCE

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