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Cofilin 1 (phospho Ser3) antibody

Anti-Cofilin 1 (phospho Ser3) antibody used in IHC (Paraffin sections) (IHC-P). GTX12866
Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX12866

Cat No. GTX12866

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Dog
Package
50 μl ($319)

APPLICATION

Application Note

IHC-P: Use at a dilution of 1/50-1/100. WB: Use at a dilution of 1/1,000. Detects a band of approximately 20 kDa. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

MDCK cells treated with staurosporine; Jurkat cells treated with hydrogen peroxide

PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.3, 50% Glycerol and 150mM NaCl containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human cofilin around the phosphorylation site of serine 3 (M-A-S^P-G-V).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cofilin 1 , Cfl , Hel-S-15 , Cofilin , Cfl1

Background

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their ‘pointed’ (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.

Research Area

DATA IMAGES

Anti-Cofilin 1 (phospho Ser3) antibody used in IHC (Paraffin sections) (IHC-P). GTX12866

GTX12866 IHC-P Image

IHC-P image using GTX12866 - Detection of Cofilin by IHC-P in human breast carcinoma tissue.

Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX12866

GTX12866 WB Image

Western blot analysis of extracts from COLO205 cells using cofilin antibody (Lane 1 and 2) and cofilin (phospho-Ser3) antibody (GTX12866, Lane 3 and 4)

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319