IHC-P: Use at a dilution of 1/50-1/100. WB: Use at a dilution of 1/1,000. Detects a band of approximately 20 kDa. Optimal dilutions/concentrations should be determined by the end user.
MDCK cells treated with staurosporine; Jurkat cells treated with hydrogen peroxide
Phosphate-buffered saline, pH 7.3, 50% Glycerol and 150mM NaCl containing 0.02% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Batch dependent (Please refer to the vial label for the specific concentration.)
The antiserum was produced against synthesized phosphopeptide derived from human cofilin around the phosphorylation site of serine 3 (M-A-S^P-G-V).
Immunogen affinity purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Cofilin 1 , Cfl , Hel-S-15 , Cofilin , Cfl1
Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their ‘pointed’ (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.