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Cofilin 1 (phospho Ser3) antibody

Orthogonal
Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX133865
Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX133865

Cat. No. GTX133865

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cofilin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

HeLa , C8D30

Predict Reactivity

Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser3 of human Cofilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887125

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cofilin 1 , CFL , HEL-S-15 , cofilin

Cellular Localization

Nucleus matrix , Cytoplasm , cytoskeleton

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX133865

GTX133865 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 (phospho Ser3) antibody (GTX133865) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX133865

GTX133865 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 (phospho Ser3) antibody (GTX133865) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Diego Lucero et al. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 2019; 1865 (2) : 158537 Interleukin 10 promotes macrophage uptake of HDL and LDL by stimulating fluid-phase endocytosis.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169