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Cofilin 1 (phospho Ser3) antibody

Orthogonal
Anti-Cofilin 1 (phospho Ser3) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50199
Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX50199
Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX50199
Anti-Cofilin 1 (phospho Ser3) antibody used in IHC (Paraffin sections) (IHC-P). GTX50199
Anti-Cofilin 1 (phospho Ser3) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50199

Cat. No. GTX50199

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Cofilin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from human Cofilin 1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11166794

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cofilin 1 , CFL , HEL-S-15 , cofilin

Cellular Localization

Cytoplasm, cytoskeleton

Background

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Anti-Cofilin 1 (phospho Ser3) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50199

GTX50199 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50199 Cofilin 1 (phospho Ser3) antibody.

Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX50199

GTX50199 WB Image

WB analysis of extracts from COLO205 cells using GTX50199 Cofilin 1 (phospho Ser3) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX50199

GTX50199 WB Image

WB analysis of extracts from various sample using GTX50199 Cofilin 1 (phospho Ser3) antibody.
Lane 1 : HeLa  treated with H2O2
Lane 2 : Mouse brain tissue
Lane 3 : Rat brain tissue
Lane 4 : N-peptide blocked HeLa  treated with H2O2
Lane 5 : N-peptide blocked Mouse brain tissue
Lane 6 : N-peptide blocked Rat brain tissue
Lane 7 : P-peptide blocked HeLa  treated with H2O2
Lane 8 : P-peptide blocked Mouse brain tissue
Lane 9 : P-peptide blocked Rat brain tissue
Loading : 40 μg per lane

Anti-Cofilin 1 (phospho Ser3) antibody used in IHC (Paraffin sections) (IHC-P). GTX50199

GTX50199 IHC-P Image

Orthogonal

IHC-P analysis of human breast carcinoma tissue using GTX50199 Cofilin 1 (phospho Ser3) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Cofilin 1 (phospho Ser3) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50199

GTX50199 ICC/IF Image

The data was published in the journal PeerJ in 2017.PMID: 28533957

REFERENCE

Application Reference

Liu X et al. PeerJ 2017; 5 : e3295 PLD2 regulates microtubule stability and spindle migration in mouse oocytes during meiotic division.
Liu X et al. PeerJ 2017; (Epub) PLD2 regulates microtubule stability and spindle migration in mouse oocytes during meiotic division.
Application : ICC/IF
Reactivity : Mouse
Wang CY et al. CLINICS ONCOL 2016; 1 : 1015 Over-Expression of Cofilin-1 Suppressed Mobility of Lung Cancer Cells is Associated with Down-Regulation of SNAIL-1 and Induction of Let-7.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399