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Cofilin 1 (phospho Ser3) antibody

Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX50199
Anti-Cofilin 1 (phospho Ser3) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50199
Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX50199
Anti-Cofilin 1 (phospho Ser3) antibody used in IHC (Paraffin sections) (IHC-P). GTX50199

Cat No. GTX50199

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from human Cofilin 1.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

RRID

AB_11166794

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cofilin 1 , CFL , HEL-S-15 , cofilin

Cellular Localization

Cytoplasm, cytoskeleton

Background

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX50199

GTX50199 WB Image

WB analysis of extracts from various sample using GTX50199 Cofilin 1 (phospho Ser3) antibody.
Lane 1 : HeLa  treated with H2O2
Lane 2 : Mouse brain tissue
Lane 3 : Rat brain tissue
Lane 4 : N-peptide blocked HeLa  treated with H2O2
Lane 5 : N-peptide blocked Mouse brain tissue
Lane 6 : N-peptide blocked Rat brain tissue
Lane 7 : P-peptide blocked HeLa  treated with H2O2
Lane 8 : P-peptide blocked Mouse brain tissue
Lane 9 : P-peptide blocked Rat brain tissue
Loading : 40 μg per lane

Anti-Cofilin 1 (phospho Ser3) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50199

GTX50199 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50199 Cofilin 1 (phospho Ser3) antibody.

Anti-Cofilin 1 (phospho Ser3) antibody used in Western Blot (WB). GTX50199

GTX50199 WB Image

WB analysis of extracts from COLO205 cells using GTX50199 Cofilin 1 (phospho Ser3) antibody.
Lane 1 : Primary antibody pre-incubated with the antigen specific peptide

Anti-Cofilin 1 (phospho Ser3) antibody used in IHC (Paraffin sections) (IHC-P). GTX50199

GTX50199 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50199 Cofilin 1 (phospho Ser3) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

REVIEW

Cofilin 1 (phospho Ser3) antibody Cat No. GTX50199

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PeerJ in 2017.PMID: 28533957
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339