GeneTex
United States (US)

Cofilin 2 antibody

GTX100213_IHC_19011506_268.jpg
GTX100213_IFA_19011506_837.jpg
GTX100213_39274_20170706_WB_M_19011506_204.jpg
GTX100213_WB_19011506_713.jpg

Cat No. GTX100213

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3 , HepG2 , Molt-4 , Raji , Neuro2A , C8D30 , Raw264.7

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cofilin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

NEM7 antibody, CFL2 antibody, "cofilin, muscle isoform antibody", cofilin-2 antibody, cofilin 2 (muscle) antibody

Cellular Localization

Nucleus matrix , Cytoplasm , cytoskeleton

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Database

DATA IMAGES
GTX100213_IHC_19011506_268.jpg

GTX100213 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Cofilin 2(GTX100213) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100213_IFA_19011506_837.jpg

GTX100213 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cofilin 2(GTX100213) antibody at 1:200 dilution.

GTX100213_39274_20170706_WB_M_19011506_204.jpg

GTX100213 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody (GTX100213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100213_WB_19011506_713.jpg

GTX100213 WB Image

Sample(30 μg of whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX100213 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 319
$ 169