GeneTex
  • Country / Location Selection

United States (US)

Cofilin 2 antibody

Anti-Cofilin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100213
Anti-Cofilin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100213
Anti-Cofilin 2 antibody used in Western Blot (WB). GTX100213
Anti-Cofilin 2 antibody used in Western Blot (WB). GTX100213

Cat No. GTX100213

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3 , HepG2 , Molt-4 , Raji , Neuro2A , C8D30 , Raw264.7

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cofilin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cofilin 2 , NEM7

Cellular Localization

Nucleus matrix , Cytoplasm , cytoskeleton

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Cofilin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX100213

GTX100213 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Cofilin 2(GTX100213) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Cofilin 2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100213

GTX100213 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cofilin 2(GTX100213) antibody at 1:200 dilution.

Anti-Cofilin 2 antibody used in Western Blot (WB). GTX100213

GTX100213 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody (GTX100213) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Cofilin 2 antibody used in Western Blot (WB). GTX100213

GTX100213 WB Image

Sample(30 μg of whole cell lysate)
A:Raji (GTX27908)
12% SDS PAGE
GTX100213 diluted at 1:1000

REFERENCE

REVIEW

There are currently no reviews for Cofilin 2 antibody (GTX100213). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 329
$ 169