GeneTex
  • Country / Location Selection

United States (US)

Coilin antibody [HL2384]

Anti-Coilin antibody [HL2384] used in Western Blot (WB). GTX638593
Anti-Coilin antibody [HL2384] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638593

Cat. No. GTX638593

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2384

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Coilin.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coilin , CLN80 , p80-coilin

Cellular Localization

Nucleus

Background

The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Coilin antibody [HL2384] used in Western Blot (WB). GTX638593

GTX638593 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Coilin antibody [HL2384] (GTX638593) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Coilin antibody [HL2384] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638593

GTX638593 ICC/IF Image

Coilin antibody [HL2384] detects Coilin protein at Cajal bodies by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: Coilin stained by Coilin antibody [HL2384] (GTX638593) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for Coilin antibody [HL2384] (GTX638593). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Coilin antibody [HL2384] (GTX638593). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations