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Col1a1a antibody

Anti-Col1a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX128243
Anti-Col1a1a antibody used in Western Blot (WB). GTX128243

Cat No. GTX128243

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Wm

Reactivity

Zebrafish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Positive Control

zebrafish muscle , whole zebrafish , zebrafish embryo

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

collagen, type I, alpha 1a , alpha1(I) , cb21 , col1a1 , sb:cb21 , sb:cb384 , wu:fa99c12 , wu:fb02c06

Database

Research Area

DATA IMAGES

Anti-Col1a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX128243

GTX128243 IHC-Wm Image

Immunofluorescent image of a cross-section of a zebrafish embryo using col1a1a (GTX128243) antibody at a 1:200 dilution. Col1a1a is localized in the blood vessels. Col1a1a(Green) a-Tubulin(Red)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-Col1a1a antibody used in Western Blot (WB). GTX128243

GTX128243 WB Image

Various tissue extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Col1a1a antibody (GTX128243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219