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Col2a1a antibody

Anti-Col2a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX127988
Anti-Col2a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX127988
Anti-Col2a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX127988
Anti-Col2a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX127988

Cat. No. GTX127988

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 IHC-Fr, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Col2a1a products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Predict Reactivity

Human (100%)| Mouse (92%)| Rat (96%)| Rabbit (100%)| Bovine (100%)| Dog (100%)| Chicken (95%)| Xenopus tropicalis (95%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Col2a1a

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11162647

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen, type II, alpha 1a , col2a1 , coll2a1 , fb38c06 , fc10c01 , wu:fb38c06 , wu:fc10c01

Database

Research Area

DATA IMAGES

Anti-Col2a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX127988

GTX127988 IHC-Wm Image

Immunofluorescent image of a zebrafish embryo eye section using col2a1a (GTX127988) antibody at a 1:200 dilution. Col2a1a (Green) a-Tubulin(Red)(This image was provided courtesy of the Schilling Lab at UC, Irvine.)

Anti-Col2a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX127988

GTX127988 IHC-Wm Image

Col2a1a antibody detects Col2a1a protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Col2a1a antibody (GTX127988) dilution: 1:100.
Anti-Col2a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX127988

GTX127988 IHC-Wm Image

Col2a1a antibody detects Col2a1a protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Col2a1a stained by Col2a1a antibody (GTX127988) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Anti-Col2a1a antibody used in IHC (Whole mount) (IHC-Wm). GTX127988

GTX127988 IHC-Wm Image

Col2a1a antibody detects Col2a1a protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Col2a1a stained by Col2a1a antibody (GTX127988) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

Application Reference

Munjal A et al. Cell 2021; 184 (26) : 6313-6325.e18 Extracellular hyaluronate pressure shaped by cellular tethers drives tissue morphogenesis
Reactivity : Zebrafish
Debanjan Mukherjee et al. Development 2021; 148 (2) Ccn2a is an injury-induced matricellular factor that promotes cardiac regeneration in zebrafish.
Application : IHC-Fr
Reactivity : Zebrafish
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169