APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human Collagen I + Collagen II + Collagen III.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
collagen type I alpha 1 chain , collagen type I alpha 2 chain , collagen type II alpha 1 chain , collagen type III alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4 , EDSARTH2 , EDSCV , OI4 , ANFH , AOM , COL11A3 , SEDC , STL1 , EDS4A , EDSVASC
Cellular Localization
Secreted, extracellular space, extracellular matrix
Database
DATA IMAGES
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GTX638633 WB Image
U87-MG whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (GTX638633) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638633 IHC-P Image
Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (GTX638633) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638633 IHC-P Image
Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (GTX638633) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638633 IHC-P Image
Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (GTX638633) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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