Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (GTX638633) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (GTX638633) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (GTX638633) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (GTX638633) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] detects secreted Collagen I + Collagen II + Collagen III protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Collagen I + Collagen II + Collagen III stained by Collagen I + Collagen II + Collagen III antibody [HL2048 + HL1907] (GTX638633) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min