Collagen I antibody (Biotin)

Cat. No. GTX26577

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS, Dot, Multiplexing
Reactivity	Human, Mouse, Rat
Package	50 μg ($399)

See all Collagen I products

Datasheet
Document

View product citations for antibody GTX26577 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:3000-1:6000
ICC/IF	Assay dependent
IHC-P	1:50-1:200
FACS	Assay dependent
Dot	Assay dependent
Multiplexing	Assay dependent

Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Product Note

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human, Bovine

Immunogen

Collagen Type I from human and bovine placenta.

Purification

Purified by antigen-affinity chromatography.
Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Conjugation

Biotin
Ratio : 1-2 molecules BAC per Rabbit IgG molecule.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4

Cellular Localization

Secreted,Extracellular space,Extracellular matrix

Background

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix, which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

Database

Gene ID: 1277 COL1A1
UniProt: P02452 COL1A1

Research Area

DATA IMAGES

GTX26577 FACS Image

Flow Cytometry of Anti-Collagen Type I Biotin Conjugated Antibody. Cells: mouse lung. Stimulation: none. Primary antibody: biotin conjugated anti-collagen type I antibody. Secondary antibody: PE-conjugated CD45 and PE-conjugated anti-collagen type I secondary antibody.

GTX26577 WB Image

Western Blot of Rabbit anti-Collagen I antibody (GTX26577). Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 μg per lane. Protein staining shown below each blot depicts equal protein loading. Primary antibody: anti-Collagen I antibody at 0.2–2 μg/10 ml for overnight at 4ºC. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 μg/10 ml for overnight at 4ºC. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band(s): none.

GTX26577 IHC-P Image

Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody (GTX26577). Tissue: Human Skin at pH9. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative control (B)

GTX26577 IHC-P Image

Immunohistochemistry of Rabbit Anti-Collagen Type I Antibody (GTX26577). Tissue: Human Skin at pH6. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Collagen Type I antibody at 10 μg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B)

GTX26577 FACS Image

Flow Cytometry of Rabbit Anti-Collagen 1 Antibody. Cells: primary adult human dermal fibroblast cells. Stimulation: none. Primary antibody: Biotin-Conjugated Collagen 1 antibody at 5μg/mL for 45 min at 4ºC. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1:500 for 15 min at RT.

GTX26577 IHC-P Image

IHC-P analysis of human skin tissue at pH 9 using GTX26577 Collagen I antibody (Biotin).
Panel A : Positive control
Panel B : Negative control
Dilution : 10 μg/mL

GTX26577 IHC-P Image

IHC-P analysis of human skin tissue at pH 6 using GTX26577 Collagen I antibody (Biotin).
Panel A : Positive control
Panel B : Negative control
Dilution : 10 μgm/mL

GTX26577 WB Image

WB analysis of Wistar rat hepatic stellate whole cell lysate using GTX26577 Collagen I antibody (Biotin).
Left lane : GFP-transduced
Right lane : PPARg-transduced
Loading : 100 μg

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package	List Price ($)
50 μg	$ 399
Qty

Collagen I antibody (Biotin)

Cat. No. GTX26577

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX26577 FACS Image

GTX26577 WB Image

GTX26577 IHC-P Image

GTX26577 IHC-P Image

GTX26577 FACS Image

GTX26577 IHC-P Image

GTX26577 IHC-P Image

GTX26577 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.