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Collagen I antibody [COL-1]

Anti-Collagen I antibody [COL-1] used in IHC (Paraffin sections) (IHC-P). GTX26308
Anti-Collagen I antibody [COL-1] used in IHC (Frozen sections) (IHC-Fr). GTX26308
Anti-Collagen I antibody [COL-1] used in IHC (Frozen sections) (IHC-Fr). GTX26308

Cat No. GTX26308

Host

Mouse

Clonality

Monoclonal

Clone Name

COL-1

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP, Dot, ELISA, IHC

Reactivity

Human, Mouse, Rat, Rabbit, Bovine, Pig, Deer
Package
100 μl ($339)
PRODUCT

Summary

Collagen I antibody (Collagen 1 antibody) detects type I collagen, a fibrillar type collagen found in almost all connective tissues. The main isoform of type I collagen is a heterotrimer of two α1 and one α2 chains subject to significant posttranslational modification and crosslinking. Mutations in type I collagen are found in osteogenesis imperfecta and Ehlers-Danlos syndrome, but this collagen is also linked to other connective tissue disease as well as disorders associated with aging.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:2000
IP Assay dependent
Dot Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Product Note

The antibody is reactive with the native (helical) form of collagen type I in ELISA and dot blot. The product is not reactive when tested on thermally denatured molecules. In immunohistochemical staining of acetone-fixed and unfixed frozen sections, a strong staining of connective tissue fibers is seen. Using the dot blot and ELISA techniques, the antibody shows no cross-reactivity with collagen types, II, III, IV, V, VI, VII, IX, X and XI. The epitope recognized by the antibody is sensitive to routine formalin fixation and paraffin embedding.

PROPERTIES

Form

Liquid

Buffer

Ascites, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Bovine

Immunogen

bovine skin collagen type I

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_385204

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

collagen type I alpha 1 chain

Background

Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for Platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor.

Database

Research Area

DATA IMAGES

Anti-Collagen I antibody [COL-1] used in IHC (Paraffin sections) (IHC-P). GTX26308

GTX26308 IHC-P Image

Collagen I antibody [COL-1] detects Collagen I protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rabbit tongue.
Collagen I stained by Collagen I antibody [COL-1] (GTX26308) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Collagen I antibody [COL-1] used in IHC (Frozen sections) (IHC-Fr). GTX26308

GTX26308 IHC-Fr Image

IHC-Fr analysis of human tongue tissue using GTX26308 Collagen I antibody [COL-1] at 1:2,000.

Anti-Collagen I antibody [COL-1] used in IHC (Frozen sections) (IHC-Fr). GTX26308

GTX26308 IHC-Fr Image

IHC-Fr analysis of pig tongue tissue using GTX26308 Collagen I antibody [COL-1] at 1:4,000.

REFERENCE

REVIEW

Collagen I antibody [COL-1] Cat No. GTX26308

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Rb
Figure Note : The data was peer reviewed and published in the journal J Orthop Surg Res in 2019.PMID: 31272458
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Pig
Figure Note : The data was peer reviewed and published in the journal Biomed Res Int in 2015.PMID: 25918720
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2020.PMID: 32235811
Application Reference
Clone Reference
SDS
Sodium Azide.pdf