GeneTex
  • Country / Location Selection

United States (US)

Collagen I antibody

Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292
Anti-Collagen I antibody used in Western Blot (WB). GTX20292
Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292

Cat No. GTX20292

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ELISA

Reactivity

Human, Mouse, Rat, Bovine, Hamster
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000 - 1:10000
ICC/IF Assay dependent
IHC-P 1:50 - 1:200
IP 1:100
ELISA 1:5000 - 1:50000

Note :

WB
This preparation results in a native conformation of the protein. Recommend not adding 2-ME or DTT in lysis buffer and not boiling the sample.

Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Product Note

Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards.  Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.   This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens.  Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

PROPERTIES

Form

Liquid

Buffer

0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA pH8.0

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Collagen Type I from human and bovine placenta by limited pepsin digestion and selective salt precipitation.

Purification

Purified by antigen-affinity chromatography.
This product was prepared by immunoaffinity chromatography on immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove unwanted specificities.

Conjugation

Unconjugated

RRID

AB_384293

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Database

Research Area

DATA IMAGES

Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292

GTX20292 IHC-P Image

IHC-P analysis of normal kidney tissue section using GTX20292 Collagen I antibody.
Antigen retrieval : No antigen retrieval was performed.
Dilution : 1:100
Localization : Distal tubules in normal kidney tissue.
Note : the absence of staining of glomeruli.

Anti-Collagen I antibody used in Western Blot (WB). GTX20292

GTX20292 WB Image

Western blot of Human Collagen Type I. Load: 50 ng Human Collagen Type 1. Primary antibody: Collagen Type I antibody (GTX20292) at 1:1,000 overnight at 4ºC. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 139 & 130 kDa.

Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292

GTX20292 IHC-P Image

IHC-P analysis of human lung tissue section using GTX20292 Collagen I antibody.
Antigen retrieval : user optimized
Dilution : 1:400
Localization : Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative.
Staining : antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

REFERENCE

REVIEW

Collagen I antibody Cat No. GTX20292

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Cell Mol Med in 2019.PMID: 30411499
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2016. PMID: 27199755
Application Reference
SDS
Sodium Azide.pdf
Package List Price ($)
$ 379