GeneTex
United States (US)

Collagen II antibody [N1], N-term

GTX100829_40751_20170510_IHC-P_M_19011506_839.jpg
GTX100829_41920_20151112_WB_19011506_925.jpg
GTX100829_40751_20170510_IHC-P_R_19011506_603.jpg

Cat No. GTX100829

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

142 kDa. ( Note )

Positive Control

HepG2 conditioned medium

Predict Reactivity

Rabbit, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Collagen II. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Collagen Type Ii Alpha 1 Chain,Anfh,Aom,Col11A3,Sedc,Stl1,Col2A1

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100829_40751_20170510_IHC-P_M_19011506_839.jpg

GTX100829 IHC-P Image

Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100829_41920_20151112_WB_19011506_925.jpg

GTX100829 WB Image

HepG2 whole cell extract and HepG2 conditioned medium (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.

GTX100829_40751_20170510_IHC-P_R_19011506_603.jpg

GTX100829 IHC-P Image

Collagen II antibody [N1], N-term detects Collagen II protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Collagen II antibody [N1], N-term (GTX100829) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169