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Collagen III antibody [C2C3-2], C-term

Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111643

Cat No. GTX111643

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

HeLa , SK-N-SH , SK-N-AS , mouse brain , rat brain

Predict Reactivity

Rat, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950012

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

collagen type III alpha 1 chain , EDS4A , EDSVASC

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Database

Research Area

DATA IMAGES

Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643

GTX111643 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III alpha1 antibody [C2C3-2], C-term (GTX111643) diluted at 1:1000.

Anti-Collagen III antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX111643

GTX111643 IHC-P Image

Collagen III antibody [C2C3-2], C-term detects Collagen III protein at secreted on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Collagen III antibody [C2C3-2], C-term (GTX111643) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Collagen III antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX111643

GTX111643 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Collagen III (GTX111643) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643

GTX111643 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3-2], C-term (GTX111643) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111643

GTX111643 ICC/IF Image

Collagen III antibody [C2C3-2], C-term detects Collagen III protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Collagen III stained by Collagen III antibody [C2C3-2], C-term (GTX111643) diluted at 1:500.

REFERENCE

REVIEW

Collagen III antibody [C2C3-2], C-term Cat No. GTX111643

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 29170419
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 29273797
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf