GeneTex

  • Country / Location Selection

United States (US)

Collagen III antibody [C2C3-2], C-term

Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111643
Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643

Cat. No. GTX111643

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Collagen III products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950012

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type III alpha 1 chain , EDS4A , EDSVASC

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Database

Research Area

DATA IMAGES

Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643

GTX111643 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III alpha1 antibody [C2C3-2], C-term (GTX111643) diluted at 1:1000.

Anti-Collagen III antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX111643

GTX111643 IHC-P Image

Collagen III antibody [C2C3-2], C-term detects Collagen III protein at secreted on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Collagen III antibody [C2C3-2], C-term (GTX111643) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Collagen III antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111643

GTX111643 ICC/IF Image

Collagen III antibody [C2C3-2], C-term detects Collagen III protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Collagen III stained by Collagen III antibody [C2C3-2], C-term (GTX111643) diluted at 1:500.

Anti-Collagen III antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX111643

GTX111643 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Collagen III (GTX111643) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643

GTX111643 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3-2], C-term (GTX111643) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [C2C3-2], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111643

GTX111643 ICC/IF Image

The data was published in the journal Sci Rep in 2017. PMID: 29170419

Anti-Collagen III antibody [C2C3-2], C-term used in Western Blot (WB). GTX111643

GTX111643 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 29273797

REFERENCE

Application Reference

Rigi M et al. Br J Ophthalmol 2024; 108 (3) : 343-348 Collagen type XII is undetectable in keratoconus Bowman's layer.
Yeh LC et al. Int J Mol Sci 2020; 21 (5) The Bioactive Core and Corona Synergism of Quantized Gold Enables Slowed Inflammation and Increased Tissue Regeneration in Wound Hypoxia.
Zheng Z et al. Front Cell Dev Biol 2020; 8 : 858 CRLF1 Is a Key Regulator in the Ligamentum Flavum Hypertrophy.
Application : WB
Reactivity : Human
Shentu TP et al. Sci Rep 2017; 7 (1) : 18052 Thy-1 dependent uptake of mesenchymal stem cell-derived extracellular vesicles blocks myofibroblastic differentiation.
Application : WB
Reactivity : Human
Saito Y et al. Sci Rep 2017; 7 (1) : 16192 PDGFR Signaling Mediates Hyperproliferation and Fibrotic Responses of Subsynovial Connective Tissue Cells in Idiopathic Carpal Tunnel Syndrome.
Application : ICC/IF
Reactivity : Human
Show More +
Submit a Reference

REVIEW

There are currently no reviews for Collagen III antibody [C2C3-2], C-term (GTX111643). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169