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Collagen III antibody [C2C3], C-term

KO/KD
Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997
Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997
Anti-Collagen III antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102997

Cat. No. GTX102997

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all Collagen III products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618014

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type III alpha 1 chain , EDS4A , EDSVASC

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Database

Research Area

DATA IMAGES

Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997

GTX102997 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (GTX102997) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997

GTX102997 WB Image

Various extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (GTX102997) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102997

GTX102997 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human PAPILLARY CA_STROMAL CELLS, using Collagen III (GTX102997) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

NN Zhong et al. BMC Oral Health 2023; 23 (1) : 454 Identification of Immune Infiltration in Odontogenic Keratocyst by Integrated Bioinformatics Analysis
Application : IHC-P
Reactivity : Human
Kai-Chieh Chou et al. Polymers (Basel) 2021; 13 (20) Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model.
Application : IHC-P
Reactivity : Rat
Chen LC et al. Cells 2021; 10 (10) Modulation of Inherent Niches in 3D Multicellular MSC Spheroids Reconfigures Metabolism and Enhances Therapeutic Potential.
Application : ICC/IF
Reactivity : Human
Mickle M et al. Mol Carcinog 2021; 60 (7) : 497-507 VGLL4 inhibits YAP1/TEAD signaling to suppress the epidermal squamous cell carcinoma cancer phenotype.
Wang C et al. Biotechnol Lett 2020; 42 (8) : 1567-1579 Mechanical injury and IL-1β regulated LOXs and MMP-1, 2, 3 expression in ACL fibroblasts co-cultured with synoviocytes.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
NN Zhong et al. BMC Oral Health 2023; 23 (1):454 Identification of Immune Infiltration in Odontogenic Keratocyst by Integrated Bioinformatics Analysis
Application : IHC-P
Reactivity : Human
Kai-Chieh Chou et al. Polymers (Basel) 2021; 13 (20) Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model.
Application : IHC-P
Reactivity : Rat
Chen LC et al. Cells 2021; 10 (10) Modulation of Inherent Niches in 3D Multicellular MSC Spheroids Reconfigures Metabolism and Enhances Therapeutic Potential.
Application : ICC/IF
Reactivity : Human
Mickle M et al. Mol Carcinog 2021; 60 (7):497-507 VGLL4 inhibits YAP1/TEAD signaling to suppress the epidermal squamous cell carcinoma cancer phenotype.
Wang C et al. Biotechnol Lett 2020; 42 (8):1567-1579 Mechanical injury and IL-1β regulated LOXs and MMP-1, 2, 3 expression in ACL fibroblasts co-cultured with synoviocytes.
Application : WB
Reactivity : Human
Chen QN et al. Exp Cell Res 2020; 389 (1):111890 Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis.
Ozawa N et al. Int J Mol Sci 2019; 20 (9) Legumain Promotes Atherosclerotic Vascular Remodeling.
Gu J et al. Evid Based Complement Alternat Med 2019; 2019:1358469 Huangqi Shengmai Yin Protects against Radiation-Induced Cardiac Fibrosis Injury by Regulating the TGF-帣1/Smads and MMPs.
Application : IHC-P
Zhang Y et al. Cell Death Dis 2019; 10 (2):124 Flavones hydroxylated at 5, 7, 3' and 4' ameliorate skin fibrosis via inhibiting activin receptor-like kinase 5 kinase activity.
Application : WB
Reactivity : Human
Han SH et al. Int J Mol Sci 2017; 18 (6) The Implication of Substance P in the Development of Tendinopathy: A Case Control Study.
Application : WB
Reactivity : Human
Schendel SA et al. Aesthet Surg J 2017; (Epub) Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics.
Application : WB
Reactivity : Human
Chen S et al. World J Urol 2016; (Epub) Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway.
Application : WB
Reactivity : Human
Ramirez-Sanchez I et al. FEBS J 2014; 281 (24):5567-80 (-)-Epicatechin improves mitochondrial-related protein levels and ameliorates oxidative stress in dystrophic δ-sarcoglycan null mouse striated muscle.
Application : WB
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169