GeneTex
United States (US)

Collagen III antibody [C2C3], C-term

KO/KD
GTX102997_42599_20181221_WB_shRNA_watermark_19011519_529.jpg
GTX102997_40100_WB_R_liver_19011506_616.jpg
GTX102997_40100_IHC_19011506_267.jpg
GTX102997_40100_WB_M_19011506_848.jpg
GTX102997_41633_20150730_WB_M_19011506_413.jpg
GTX102997_40100_WB_R_19011506_346.jpg
GTX102997_42599_20170112_WB_19011506_745.jpg

Cat No. GTX102997

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

HeLa , COL3A1 shRNA-transfected HeLa , SK-N-SH , SK-N-AS , Neuro2A , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , Mouse ESC , PC-12 , Rat2 , rat liver

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EDS4A antibody, FLJ34534 antibody, COL3A1 antibody, alpha1 (III) collagen antibody, Ehlers-Danlos syndrome type IV, autosomal dominant antibody, collagen, fetal antibody, collagen alpha-1(III) chain antibody, collagen, type III, alpha 1 antibody

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Database

DATA IMAGES
GTX102997_42599_20181221_WB_shRNA_watermark_19011519_529.jpg

GTX102997 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (GTX102997) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102997_40100_WB_R_liver_19011506_616.jpg

GTX102997 WB Image

Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis.
A. 50 µg rat liver lysate/extract
5% SDS-PAGE
COL3A1 antibody [C2C3], C-term (GTX102997) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102997_40100_IHC_19011506_267.jpg

GTX102997 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human PAPILLARY CA_STROMAL CELLS, using Collagen III (GTX102997) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102997_40100_WB_M_19011506_848.jpg

GTX102997 WB Image

Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis.
A. 30 µg Neuro2A whole lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysate/extract
D. 30 µg Raw264.7 whole cell lysate/extract
E. 30 µg C2C12 whole cell lysate/extract
5% SDS-PAGE
Collagen III antibody [C2C3], C-term (GTX102997) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102997_41633_20150730_WB_M_19011506_413.jpg

GTX102997 WB Image

Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. Mouse tissue extracts (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (GTX102997) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102997_40100_WB_R_19011506_346.jpg

GTX102997 WB Image

Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis.
A. 30 µg PC-12 whole cell extract
B. 30 µg Rat2 whole cell extract
5% SDS-PAGE
Collagen III antibody [C2C3], C-term (GTX102997) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102997_42599_20170112_WB_19011506_745.jpg

GTX102997 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (GTX102997) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169