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Collagen III antibody [C2C3], C-term

Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997
Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997
Anti-Collagen III antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102997
Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997
Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997
Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997
Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997

Cat No. GTX102997

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Positive Control

HeLa , COL3A1 shRNA-transfected HeLa , SK-N-SH , SK-N-AS , Neuro2A , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , Mouse ESC , PC-12 , Rat2 , rat liver

Predict Reactivity

Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Collagen III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

collagen type III alpha 1 chain , EDS4A , EDSVASC

Cellular Localization

Secreted , extracellular space , extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]

Database

Research Area

DATA IMAGES

Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997

GTX102997 WB Image

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (GTX102997) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997

GTX102997 WB Image

Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis.
A. 50 μg rat liver lysate/extract
5% SDS-PAGE
COL3A1 antibody [C2C3], C-term (GTX102997) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102997

GTX102997 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human PAPILLARY CA_STROMAL CELLS, using Collagen III (GTX102997) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997

GTX102997 WB Image

Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis.
A. 30 μg Neuro2A whole lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
E. 30 μg C2C12 whole cell lysate/extract
5% SDS-PAGE
Collagen III antibody [C2C3], C-term (GTX102997) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997

GTX102997 WB Image

Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis. Mouse tissue extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (GTX102997) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997

GTX102997 WB Image

Collagen III antibody [C2C3], C-term detects Collagen III protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
B. 30 μg Rat2 whole cell extract
5% SDS-PAGE
Collagen III antibody [C2C3], C-term (GTX102997) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [C2C3], C-term used in Western Blot (WB). GTX102997

GTX102997 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (GTX102997) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169