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Collagen III antibody [HL1906]

Recombinant antibody Orthogonal
Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655
Anti-Collagen III antibody [HL1906] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637655
Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655
Anti-Collagen III antibody [HL1906] used in IHC (Paraffin sections) (IHC-P). GTX637655

Cat. No. GTX637655

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1906

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Collagen III products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Product Note

This antibody is specific for human COL3A1 protein, and it does not cross react with human COL2A1, COL1A2, and COL1A1 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Collagen III

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type III alpha 1 chain , EDS4A , EDSVASC

Cellular Localization

Secreted, extracellular space, extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Database

Research Area

DATA IMAGES

Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655

GTX637655 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (GTX637655) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Collagen III antibody [HL1906] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637655

GTX637655 ICC/IF Image

Collagen III antibody [HL1906] detects Collagen III protein at cytoplasmic vesicle by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.
Green: Collagen III stained by Collagen III antibody [HL1906] (GTX637655) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Collagen III antibody [HL1906] used in Western Blot (WB). GTX637655

GTX637655 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (GTX637655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Collagen III antibody [HL1906] used in IHC (Paraffin sections) (IHC-P). GTX637655

GTX637655 IHC-P Image

Collagen III antibody [HL1906] detects Collagen III protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
Collagen III stained by Collagen III antibody [HL1906] (GTX637655) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

W Bo et al. Adv Sci (Weinh) 2024; : e2305581 Manipulation of Glutamatergic Neuronal Activity in the Primary Motor Cortex Regulates Cardiac Function in Normal and Myocardial Infarction Mice
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Package List Price ($)
$ 399
$ 169
Custom Conjugations