Collagen III antibody [HL1906]

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (GTX637655) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Collagen III antibody [HL1906] detects Collagen III protein at cytoplasmic vesicle by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min.
Green: Collagen III stained by Collagen III antibody [HL1906] (GTX637655) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (GTX637655) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.