APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1,000-1:5000 |
| Assay dependent |
| 1:100 |
| 1:10,000-1:50,000 |
Not tested in other applications.
Calculated MW
Specificity/Sensitivity
This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
PROPERTIES
Form
Liquid
Buffer
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride pH 7.2, 0.01% (w/v) Thimerosal, 10 mg/mL BSA (Immunoglobulin and Protease free)
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Collagen Type III from human and bovine placenta.
Purification
Purified by antigen-affinity chromatography
Conjugation
Horseradish peroxidase(HRP)
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
collagen type III alpha 1 chain , EDS4A , EDSVASC
Cellular Localization
Extracellular matrix
Background
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
Database
Research Area
DATA IMAGES
|
GTX00777 IHC-P Image
IHC-P analysis of human skin (left, dermis) and testis (right) using GTX00777 Collagen III antibody (HRP). The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining.
Antigen retrieval : 0.01 M sodium citrate buffer, pH 6.0 at 99-100ºC with 20 minutes
Dilution : 1:400
|
REFERENCE
There are currently no references for Collagen III antibody (HRP) (GTX00777). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Collagen III antibody (HRP) (GTX00777). Be the first to share your experience with this product.
