Collagen III antibody (HRP)

Cat. No. GTX00777

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IP, ELISA
Reactivity	Human, Bovine
Package	50 μg ($599)

See all Collagen III products

Datasheet
Document

View product citations for antibody GTX00777 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:5000
IHC-P	1:200-1:1000
IP	1:100
ELISA	1:10000-1:50000

Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Product Note

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has expected cross-reactivity with Type I and negligible cross reactivity with Type II, IV, V or VI collagens.

PROPERTIES

Form

Liquid

Buffer

10mM Sodium Phosphate, 250mM NaCl, 1% BSA

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human, Bovine

Immunogen

Collagen Type III from human and bovine placenta.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type III alpha 1 chain , EDS4A , EDSVASC

Cellular Localization

Extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Database

Gene ID: 1281 COL3A1
UniProt: P02461 COL3A1

Research Area

DATA IMAGES

GTX00777 IHC-P Image

IHC-P analysis of human skin (left, dermis) and testis (right) using GTX00777 Collagen III antibody (HRP). The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining.
Antigen retrieval : 0.01 M sodium citrate buffer, pH 6.0 at 99-100ºC with 20 minutes
Dilution : 1:400

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SDS
Thimerosal.pdf

Package

List Price ($)

50 μg

$ 599

Qty