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Collagen III antibody (HRP)

Anti-Collagen III antibody (HRP) used in IHC (Paraffin sections) (IHC-P). GTX00777

Cat No. GTX00777

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ELISA

Reactivity

 Human, Bovine
Package
50 μg ($439)
See all Collagen III products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000-1:5000
IHC-P Assay dependent
IP 1:100
ELISA 1:10,000-1:50,000
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

PROPERTIES

Form

Liquid

Buffer

0.01 M Sodium Phosphate, 0.25 M Sodium Chloride pH 7.2, 0.01% (w/v) Thimerosal, 10 mg/mL BSA (Immunoglobulin and Protease free)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Collagen Type III from human and bovine placenta.

Purification

Purified by antigen-affinity chromatography

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

collagen type III alpha 1 chain , EDS4A , EDSVASC

Cellular Localization

Extracellular matrix

Background

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Database

Research Area

DATA IMAGES

Anti-Collagen III antibody (HRP) used in IHC (Paraffin sections) (IHC-P). GTX00777

GTX00777 IHC-P Image

IHC-P analysis of human skin (left, dermis) and testis (right) using GTX00777 Collagen III antibody (HRP). The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining.
Antigen retrieval : 0.01 M sodium citrate buffer, pH 6.0 at 99-100ºC with 20 minutes
Dilution : 1:400

REFERENCE

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Package List Price ($)
$ 439