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Collagen IV antibody [COL-94]

Anti-Collagen IV antibody [COL-94] used in IHC (Paraffin sections) (IHC-P). GTX26311
Anti-Collagen IV antibody [COL-94] used in IHC (Paraffin sections) (IHC-P). GTX26311

Cat No. GTX26311

Host Mouse
Clonality Monoclonal
Clone Name COL-94
Isotype IgG1
Application WB, ICC/IF, IHC-P, IHC-Fr, Dot, ELISA, EM
Reactivity Human
Package
100 μl ($349)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:500
IHC-Fr Assay dependent
Dot Assay dependent
ELISA Assay dependent
EM Assay dependent
Not tested in other applications.

Specificity/Sensitivity

Using immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the α1 and/or α2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C.
This product does not react with Rat,Rabbit,Goat,Sheep,Cat,Chicken.
PROPERTIES

Form

Liquid

Buffer

Ascites, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

human collagen type IV

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

Research Area

DATA IMAGES
Anti-Collagen IV antibody [COL-94] used in IHC (Paraffin sections) (IHC-P). GTX26311

GTX26311 IHC-P Image

IHC-P analysis of human placenta sections using GTX26311 Collagen IV[COL-94] antibody at 1:500.

Anti-Collagen IV antibody [COL-94] used in IHC (Paraffin sections) (IHC-P). GTX26311

GTX26311 IHC-P Image

IHC-P analysis of human tongue tissue using GTX26311 Collagen IV antibody [COL-94] at 1:500.

REFERENCE
REVIEW
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Package List Price ($)
$ 349