GeneTex

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United States (US)

Collagen IV antibody

Cat. No. GTX19808

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, ELISA, RIA

Reactivity

 Mouse
Package
100 μl ($399)
See all Collagen IV products

APPLICATION

Application Note

ELISA: 1/200. IF: 1/80. Use on frozen mouse tissues (skin, liver). IHC-P: 1/500. RIA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.

Product Note

Recognises Mouse Collagen Type IV (100% by RIA). Does not react with Mouse Collagen Types I or III, Human Collagen Type IV or V, Mouse Fibronectin or Mouse Laminin (<0.1% by RIA).
We do not recommend use of this product for Human samples.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Full length native protein (extracted and purified from tumor tissues) (Mouse).

Purification

Purified
Ion exchange chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Secreted (Basement membrane).

Background

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

Research Area

REFERENCE

Application Reference

Philipp-Sebastian Koch et al. Front Physiol 2021; 12 : 722394 Imbalanced Activation of Wnt-/β-Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation.
Application : IHC-P
Reactivity : Mouse
Heil J et al. Nat Commun 2021; 12 (1) : 6963 Bone marrow sinusoidal endothelium controls terminal erythroid differentiation and reticulocyte maturation.
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Package List Price ($)
$ 399