ELISA: 1/1000. IHC-Fr: 1/20 if FITC-labeled secondary antibodies are used. Otherwise use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: ~180 kDa. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.
GTX19812 reacts with native human as well as heat denatured human collagen type V in RIA, ELISA and immunoblot/WB assays. Does not react with other collagen types I, II, III, and IV. Human plasma proteins do not interfere with binding to collagen.
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Full length native protein (purified) (Human).
PEG6000 precipitation and Sepharose 6B gel filtration.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.
GTX19812 IHC Image