Collagen V antibody [MC5-HA]

Cat. No. GTX19812

Host	Mouse
Clonality	Monoclonal
Clone Name	MC5-HA
Isotype	IgM
Application	WB, IHC-Fr, ELISA, IHC (Formalin), IHC, RIA
Reactivity	Human
Package	500 μl ($399)

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Datasheet

View product citations for antibody GTX19812 on CiteAb

APPLICATION

Application Note

ELISA: 1/1000. IHC-Fr: 1/20 if FITC-labeled secondary antibodies are used. Otherwise use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: ~180 kDa. Is unsuitable for IHC-P. Optimal dilutions/concentrations should be determined by the end user.

Product Note

GTX19812 reacts with native human as well as heat denatured human collagen type V in RIA, ELISA and immunoblot/WB assays. Does not react with other collagen types I, II, III, and IV. Human plasma proteins do not interfere with binding to collagen.

PROPERTIES

Form

Liquid

Buffer

0.01M Sodium phosphate pH 8, 0.01M Sodium borate, 0.15M Sodium chloride, 1% Dextran, 1% Mannitol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length native protein (purified) (Human).

Purification

Purified IgM
PEG6000 precipitation and Sepharose 6B gel filtration.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cytoplasmic

Background

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Collagen V is composed of several subunits as follows: trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Type V Collagen is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue specific chain combinations. Mutations in this gene are associated with Ehlers Danlos syndrome, types I and II.

Research Area

DATA IMAGES

GTX19812 IHC Image

REFERENCE

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REVIEW

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Package

List Price ($)

500 μl

$ 399

Qty

Collagen V antibody [MC5-HA]

Cat. No. GTX19812

Host

Clonality

Clone Name

Isotype

Application

Reactivity