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United States (US)

Collagen V antibody

Cat. No. GTX44061

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr, ELISA

Reactivity

Human, Bovine
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5,000-1:10,000
IHC-Fr 1:50-1:200
ELISA Assay dependent

Note :

WB
using non-reducing conditions

Not tested in other applications.

Product Note

Reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV, and VIcollagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagenextracellular matrix proteins is negligible. Typically less than 1% cross reactivity against other types of collagen wasdetected using ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffinembedded tissues.

PROPERTIES

Form

Liquid

Buffer

0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA pH8.0

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Collagen Type V from human and bovine placenta.

Purification

Purified by Affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399