Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:5,000-1:10,000 |
1:50-1:200 |
Assay dependent |
Note :
WB
using non-reducing conditions
|
Not tested in other applications.
Product Note
Reacts with most mammalian Type V collagens and has negligible cross-reactivity with Type I, II, III, IV, and VIcollagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagenextracellular matrix proteins is negligible. Typically less than 1% cross reactivity against other types of collagen wasdetected using ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffinembedded tissues.
Form
Liquid
Buffer
0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA pH8.0
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Collagen Type V from human and bovine placenta.
Purification
Purified by Affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.