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Collagen VII antibody, Internal

Anti-Collagen VII antibody, Internal used in Western Blot (WB). GTX89040

Cat. No. GTX89040

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Rat
Package
100 μg ($399)
See all Collagen VII products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-1.5μg/ml
Not tested in other applications.

Calculated MW

295 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89040-PEP).

Predict Reactivity

Human, Mouse, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TVQYSDDPRTEF, from the internal region of the protein sequence according to NP_000085.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type VII alpha 1 chain , EBD1 , EBDCT , EBR1 , NDNC8

Background

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Collagen VII antibody, Internal used in Western Blot (WB). GTX89040

GTX89040 WB Image

WB analysis of rat testis lysate using GTX89040 Collagen VII antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399