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Collagen X antibody

Orthogonal
Anti-Collagen X antibody used in IHC (Paraffin sections) (IHC-P). GTX37732
Anti-Collagen X antibody used in Western Blot (WB). GTX37732
Anti-Collagen X antibody used in Western Blot (WB). GTX37732
Anti-Collagen X antibody used in IHC (Paraffin sections) (IHC-P). GTX37732
Anti-Collagen X antibody used in Western Blot (WB). GTX37732

Cat. No. GTX37732

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC

Reactivity

 Human, Mouse, Rat, Rabbit
Package
100 μg ($459)
See all Collagen X products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-2000
IHC-P 1:300-500
IHC Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived from human Collagen X.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_11171369

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type X alpha 1 chain

Background

This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Collagen X antibody used in IHC (Paraffin sections) (IHC-P). GTX37732

GTX37732 IHC-P Image

IHC-P analysis of rat liver tissue using GTX37732 Collagen X antibody.
Dilution : 2.5μg/ml

Anti-Collagen X antibody used in Western Blot (WB). GTX37732

GTX37732 WB Image

WB analysis of mouse pancreas (Lane 1), human breast cancer (Lane 2), human mammary fibroma (Lane 3) lysates using GTX37732 Collagen X antibody.
Dilution : 1μg/ml

Anti-Collagen X antibody used in Western Blot (WB). GTX37732

GTX37732 WB Image

WB analysis of mouse spinal cord (Lane 1), mouse liver (Lane 2), mouse pancreas (Lane 3), human breast cancer (Lane 4), human mammary fibroma (Lane 5) lysates using GTX37732 Collagen X antibody.
Dilution : 1μg/ml

Anti-Collagen X antibody used in IHC (Paraffin sections) (IHC-P). GTX37732

GTX37732 IHC-P Image

IHC-P analysis of mouse bone marrow tissue using GTX37732 Collagen X antibody.
Dilution : 2.5μg/ml

Anti-Collagen X antibody used in Western Blot (WB). GTX37732

GTX37732 WB Image

Orthogonal

The data was published in the journal Biomed Res Int in 2018. PMID: 29992158

REFERENCE

Application Reference

Y Zhang et al. Ann Anat 2024; 253 : 152224 Longitudinal skeletal growth and growth plate morphological characteristics of chondro-tissue specific CUL7 knockout mice
Application : WB, IHC-P
H Suito et al. Sci Rep 2023; Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage
Pei YA et al. Mater Today Bio 2023; 23 : 100805 The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation.
YA Pei et al. Mater Today Bio 2023; : 100805 The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation
Pei M et al. Bioact Mater 2023; 23 : 353-367 Matrix from urine stem cells boosts tissue-specific stem cell mediated functional cartilage reconstruction.
Application : IHC
Reactivity : Rabbit
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REVIEW

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Application Reference
Y Zhang et al. Ann Anat 2024; 253:152224 Longitudinal skeletal growth and growth plate morphological characteristics of chondro-tissue specific CUL7 knockout mice
Application : WB, IHC-P
H Suito et al. Sci Rep 2023; Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage
Pei YA et al. Mater Today Bio 2023; 23:100805 The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation.
YA Pei et al. Mater Today Bio 2023;:100805 The matrix microenvironment influences but does not dominate tissue-specific stem cell lineage differentiation
Pei M et al. Bioact Mater 2023; 23:353-367 Matrix from urine stem cells boosts tissue-specific stem cell mediated functional cartilage reconstruction.
Application : IHC
Reactivity : Rabbit
Camacho P et al. J Biol Methods 2021; 8 (1):e146 Fabricating spatially functionalized 3D-printed scaffolds for osteochondral tissue engineering.
Nasi S et al. Front Cell Dev Biol 2021; 9:612440 Xanthine Oxidoreductase Is Involved in Chondrocyte Mineralization and Expressed in Osteoarthritic Damaged Cartilage.
Application : IHC
Horita M et al. Calcif Tissue Int 2019; (Epub) Involvement of ADAM12 in Chondrocyte Differentiation by Regulation of TGF-β1-Induced IGF-1 and RUNX-2 Expressions.
Application : IHC-P
Reactivity : Mouse
Shi Y et al. Nat Commun 2019; 10 (1):1914 A small molecule promotes cartilage extracellular matrix generation and inhibits osteoarthritis development.
Application : IHC-P
Reactivity : Mouse
Shi Z et al. Biomed Res Int 2018; 2018:6947612 Condylar Degradation from Decreased Occlusal Loading following Masticatory Muscle Atrophy.
Zeira O et al. Stem Cells Transl Med 2018; (Epub) Intra-Articular Administration of Autologous Micro-Fragmented Adipose Tissue in Dogs with Spontaneous Osteoarthritis: Safety, Feasibility, and Clinical Outcomes.
Application : IHC-P
Reactivity : Human
Palagano E et al. Bone 2018; 114:125-136 Mutations in the Neuroblastoma Amplified Sequence gene in a family affected by Acrofrontofacionasal Dysostosis type 1.
Application : IHC-P
Reactivity : Mouse
Zhan Shi et al. BioMed Research International 2018; 2018:Article ID 6947612 Condylar Degradation from Decreased Occlusal Loading following Masticatory Muscle Atrophy.
Application : WB
Reactivity : Rat
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459