GeneTex
  • Country / Location Selection

United States (US)

Contactin 1 antibody

Anti-Contactin 1 antibody used in Western Blot (WB). GTX133205
Anti-Contactin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133205

Cat No. GTX133205

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-Fr

Reactivity

Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of mouse Contactin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

contactin 1 , AW495098 , CNTN , F3cam , usl

Cellular Localization

Cell membrane , GPI-anchor

Background

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.

Database

Research Area

DATA IMAGES

Anti-Contactin 1 antibody used in Western Blot (WB). GTX133205

GTX133205 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Contactin 1 antibody (GTX133205) diluted at 1:1000.

Anti-Contactin 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133205

GTX133205 IHC-Fr Image

Contactin 1 antibody detects Contactin 1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: Contactin 1 protein stained by Contactin 1 antibody (GTX133205) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for Contactin 1 antibody (GTX133205). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Contactin 1 antibody (GTX133205). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169