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Coronin 1A antibody [C3], C-term

GTX106424_39715_20170601_IHC-P_M_1_19011506_251.jpg
GTX106424_39715_20161027_WB_19011506_604.jpg
GTX106424_39715_20170831_IHC-Fr_M_19011506_497.jpg
GTX106424_39715_20170601_IHC-P_R_1_19011506_529.jpg
GTX106424_39715_IHC_19011506_344.jpg
GTX106424_39715_20171115_ICC_IF_R_19011506_182.jpg
GTX106424_39715_20170601_IHC-P_R_2_19011506_701.jpg
GTX106424_39715_20170601_IHC-P_M_2_19011506_565.jpg

Cat No. GTX106424

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Coronin 1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CLABP antibody, CLIPINA antibody, FLJ41407 antibody, HCORO1 antibody, MGC117380 antibody, TACO antibody, p57 antibody, CORO1A antibody, coronin-1A antibody, tryptophan aspartate-containing coat protein antibody, coronin-like protein p57 antibody, coronin-like protein A antibody, clipin-A antibody, coronin-1 antibody, "coronin, actin binding protein, 1A antibody"

Cellular Localization

Cytoplasm , cytoskeleton , cell cortex , Cytoplasmic vesicle , phagosome membrane

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX106424_39715_20170601_IHC-P_M_1_19011506_251.jpg

GTX106424 IHC-P Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106424_39715_20161027_WB_19011506_604.jpg

GTX106424 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:1000.

GTX106424_39715_20170831_IHC-Fr_M_19011506_497.jpg

GTX106424 IHC-Fr Image

Coronin 1A antibody [C3], C-term detects Coronin 1A Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: Coronin 1A stained by Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX106424_39715_20170601_IHC-P_R_1_19011506_529.jpg

GTX106424 IHC-P Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106424_39715_IHC_19011506_344.jpg

GTX106424 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Coronin 1A(GTX106424) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106424_39715_20171115_ICC_IF_R_19011506_182.jpg

GTX106424 ICC/IF Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Coronin 1A stained by Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX106424_39715_20170601_IHC-P_R_2_19011506_701.jpg

GTX106424 IHC-P Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX106424_39715_20170601_IHC-P_M_2_19011506_565.jpg

GTX106424 IHC-P Image

Coronin 1A antibody [C3], C-term detects Coronin 1A protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Coronin 1A antibody [C3], C-term (GTX106424) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf