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Coronin 1A antibody [N2C2], Internal

Anti-Coronin 1A antibody [N2C2], Internal used in Western Blot (WB). GTX113932
Anti-Coronin 1A antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113932
Anti-Coronin 1A antibody [N2C2], Internal used in Western Blot (WB). GTX113932

Cat. No. GTX113932

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Coronin 1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

NCI-H929 , mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coronin 1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036646

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coronin 1A , CLABP , CLIPINA , HCORO1 , IMD8 , TACO , p57

Cellular Localization

Cytoplasm , cytoskeleton , cell cortex , Cytoplasmic vesicle , phagosome membrane

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Coronin 1A antibody [N2C2], Internal used in Western Blot (WB). GTX113932

GTX113932 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1A antibody [N2C2], Internal (GTX113932) diluted at 1:500.

Anti-Coronin 1A antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX113932

GTX113932 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CAL27 xenograft, using Coronin 1A(GTX113932) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Coronin 1A antibody [N2C2], Internal used in Western Blot (WB). GTX113932

GTX113932 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1A antibody [N2C2], Internal (GTX113932) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169