GeneTex
United States (US)

Coronin 1A antibody

Overexpression
GTX14787_386_IHC-P_18121410_173.jpg
GTX14787_2588_WB_18121410_759.jpg
GTX14787_385_IHC-P_18121410_850.jpg
GTX14787_1402_WB_18121410_513.jpg

Cat No. GTX14787

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX14787-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-KRLDRLEETVQAK, from the C Terminus of the protein sequence according to NP_009005.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Coronin 1A,Clabp,Clipina,Hcoro1,Imd8,Taco,P57,Coro1A

Cellular Localization

In noninfected macrophages, associated with the cortical microtubule network; in mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly depend

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]

Database

Research Area

DATA IMAGES
GTX14787_386_IHC-P_18121410_173.jpg

GTX14787 IHC-P Image

IHC-P analysis of human spleen using GTX14787 Coronin 1A antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX14787_2588_WB_18121410_759.jpg

GTX14787 WB Image

Overexpression

WB analysis of HEK293 overexpressing Coronin 1A (mock transfection in first lane) using GTX14787 Coronin 1A antibody.

GTX14787_385_IHC-P_18121410_850.jpg

GTX14787 IHC-P Image

IHC-P analysis of human small intestine using GTX14787 Coronin 1A antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX14787_1402_WB_18121410_513.jpg

GTX14787 WB Image

WB analysis of human lymphoblastoid using GTX14787 Coronin 1A antibody.
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 319