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Coronin 1B antibody [3D10]

Overexpression
Anti-Coronin 1B antibody [3D10] used in Western Blot (WB). GTX84676
Anti-Coronin 1B antibody [3D10] used in Western Blot (WB). GTX84676
Anti-Coronin 1B antibody [3D10] used in Western Blot (WB). GTX84676
Anti-Coronin 1B antibody [3D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84676
Anti-Coronin 1B antibody [3D10] used in Flow cytometry (FACS). GTX84676

Cat No. GTX84676

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D10

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Mouse
Package
100 μl ($419)
See all Coronin 1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.64 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CORO1B (NP_065174) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

coronin 1B , CORONIN-2

Cellular Localization

Cytoplasm, cytoskeleton

Background

Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.

Database

Research Area

DATA IMAGES

Anti-Coronin 1B antibody [3D10] used in Western Blot (WB). GTX84676

GTX84676 WB Image

WB analysis of various samples using GTX84676 Coronin 1B antibody [3D10].
Loading : 10 ug per lane
Dilution : 1:200

Anti-Coronin 1B antibody [3D10] used in Western Blot (WB). GTX84676

GTX84676 WB Image

WB analysis of various cell lines using GTX84676 Coronin 1B antibody [3D10].
Loading : 35 ug per lane

Anti-Coronin 1B antibody [3D10] used in Western Blot (WB). GTX84676

GTX84676 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Coronin 1B plasmid (Right) or empty vector (Left) for 48 hrs using GTX84676 Coronin 1B antibody [3D10].
Loading : 5 ug per lane

Anti-Coronin 1B antibody [3D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84676

GTX84676 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Coronin 1B plasmid using GTX84676 Coronin 1B antibody [3D10].

Anti-Coronin 1B antibody [3D10] used in Flow cytometry (FACS). GTX84676

GTX84676 FACS Image

FACS analysis of HEK293T cells transfected with either Coronin 1B plasmid(Red) or empty vector control plasmid(Blue) using GTX84676 Coronin 1B antibody [3D10].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419