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Coronin 1C antibody [C1C3]

Anti-Coronin 1C antibody [C1C3] used in Western Blot (WB). GTX115727
Anti-Coronin 1C antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115727
Anti-Coronin 1C antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115727
Anti-Coronin 1C antibody [C1C3] used in Western Blot (WB). GTX115727
Anti-Coronin 1C antibody [C1C3] used in Western Blot (WB). GTX115727

Cat. No. GTX115727

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Coronin 1C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HeLa , NIH-3T3 , Mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Coronin 1C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624547

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coronin 1C , HCRNN4

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Coronin 1C antibody [C1C3] used in Western Blot (WB). GTX115727

GTX115727 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX115727 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Coronin 1C antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115727

GTX115727 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Coronin 1C(GTX115727) antibody at 1:500 dilution.

Anti-Coronin 1C antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX115727

GTX115727 IHC-P Image

Coronin 1C antibody [C1C3] detects Coronin 1C protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Coronin 1C antibody [C1C3] (GTX115727) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Coronin 1C antibody [C1C3] used in Western Blot (WB). GTX115727

GTX115727 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX115727 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Coronin 1C antibody [C1C3] used in Western Blot (WB). GTX115727

GTX115727 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX115727 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169