GeneTex
United States (US)

Coronin 1C antibody [C1C3]

GTX115727_40296_WB_M_brain_18121410_885.jpg
GTX115727_40296_20141205_IHC_18121410_240.jpg
GTX115727_40296_WB_18121410_981.jpg
GTX115727_40296_WB_M_18121410_387.jpg
GTX115727_40296_IFA_18121410_974.jpg

Cat No. GTX115727

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , NIH-3T3 , mouse brain

Predict Reactivity

Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Coronin 1C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Coronin 1C,Hcrnn4,Coro1C

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115727_40296_WB_M_brain_18121410_885.jpg

GTX115727 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX115727 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115727_40296_20141205_IHC_18121410_240.jpg

GTX115727 IHC-P Image

Coronin 1C antibody [C1C3] detects Coronin 1C protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Coronin 1C antibody [C1C3] (GTX115727) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115727_40296_WB_18121410_981.jpg

GTX115727 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX115727 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115727_40296_WB_M_18121410_387.jpg

GTX115727 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
GTX115727 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115727_40296_IFA_18121410_974.jpg

GTX115727 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Coronin 1C(GTX115727) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169