APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , 293T , A431 , HeLa , HepG2
Predict Reactivity
Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Coronin 1C. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240646
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
coronin 1C , HCRNN4
Background
This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469](/templates/default/js/lightbox/images/loading.gif "Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469")
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GTX106469 WB Image
Wild-type (WT) and Coronin 1C knockout (KO) HEK293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term (GTX106469) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106469 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term (GTX106469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX106469 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using Coronin 1C(GTX106469) antibody at 1:200 dilution.
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GTX106469 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term (GTX106469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX106469 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human Sarcoma meta to brain Blood Vessel, using CORO1C (GTX106469) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for Coronin 1C antibody [C3], C-term (GTX106469). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Coronin 1C antibody [C3], C-term (GTX106469). Be the first to share your experience with this product.
![Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469](/upload/website/prouct_img/small/GTX106469/GTX106469_40800_20181102_WB_KO_watermark_w_23060120_781.webp "Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469")
![Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469](/upload/website/prouct_img/small/GTX106469/GTX106469_40800_20181026_WB_M_w_23060120_778.webp "Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469")
![Anti-Coronin 1C antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106469](/upload/website/prouct_img/small/GTX106469/GTX106469_39708_IFA_w_23060120_354.webp "Anti-Coronin 1C antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106469")
![Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469](/upload/website/prouct_img/small/GTX106469/GTX106469_40800_20181228_WB_w_23060120_691.webp "Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469")
![Anti-Coronin 1C antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106469](/upload/website/prouct_img/small/GTX106469/GTX106469_IHC_w_23060120_931.webp "Anti-Coronin 1C antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106469")
![Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469](/upload/website/prouct_img/normal/GTX106469/GTX106469_40800_20181102_WB_KO_watermark_w_23060120_781.webp "Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469")
![Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469](/upload/website/prouct_img/normal/GTX106469/GTX106469_40800_20181026_WB_M_w_23060120_778.webp "Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469")
![Anti-Coronin 1C antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106469](/upload/website/prouct_img/normal/GTX106469/GTX106469_39708_IFA_w_23060120_354.webp "Anti-Coronin 1C antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106469")
![Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469](/upload/website/prouct_img/normal/GTX106469/GTX106469_40800_20181228_WB_w_23060120_691.webp "Anti-Coronin 1C antibody [C3], C-term used in Western Blot (WB). GTX106469")
![Anti-Coronin 1C antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106469](/upload/website/prouct_img/normal/GTX106469/GTX106469_IHC_w_23060120_931.webp "Anti-Coronin 1C antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX106469")