GeneTex
United States (US)

Coronin 1C antibody [C3], C-term

KO/KD
GTX106469_40800_20181102_WB_KO_watermark_19011519_758.jpg
GTX106469_40800_20181228_WB_19011519_361.jpg
GTX106469_40800_20181026_WB_M_19011519_528.jpg
GTX106469_IHC_19011506_208.jpg
GTX106469_39708_IFA_19011506_796.jpg

Cat No. GTX106469

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Coronin 1C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HCRNN4 antibody, CORO1C antibody, coronin-1C antibody, coronin 1C antibody, coronin-3 antibody, "coronin, actin-binding protein, 1C antibody", "coronin, actin binding protein, 1C antibody"

Background

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX106469_40800_20181102_WB_KO_watermark_19011519_758.jpg

GTX106469 WB Image

KO/KD

Wild-type (WT) and Coronin 1C knockout (KO) HEK293T cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term (GTX106469) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX106469_40800_20181228_WB_19011519_361.jpg

GTX106469 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term (GTX106469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX106469_40800_20181026_WB_M_19011519_528.jpg

GTX106469 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term (GTX106469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX106469_IHC_19011506_208.jpg

GTX106469 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human Sarcoma meta to brain Blood Vessel, using CORO1C (GTX106469) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX106469_39708_IFA_19011506_796.jpg

GTX106469 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Coronin 1C(GTX106469) antibody at 1:200 dilution.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169