APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.01-0.03μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX21374-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence WKASAGHAVSIA-C, from the N Terminus of the protein sequence according to NP_005222.2; NP_612632.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cortactin , EMS1
Cellular Localization
Cytoplasm, cytoskeleton , Cell projection, dendrite , Cell membrane , Cell junction
Background
This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]
Database
Research Area
DATA IMAGES
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GTX21374 WB Image
WB analysis of human brain (cerebellum) lysate using GTX21374 Cortactin antibody, N-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX21374 WB Image
WB analysis of HEK293 cell lysate using GTX21374 Cortactin antibody, N-term.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer
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GTX21374 WB Image
WB analysis of mouse brain lysate using GTX21374 Cortactin antibody, N-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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