GeneTex
United States (US)

Cortactin antibody [N1], N-term

KO/KD
GTX100253_39568_20170309_WB_KO_watermark_19011506_807.jpg
GTX100253_39568_WB_M_19011506_162.jpg
GTX100253_39568_20170518_WB_M_R_19011506_210.jpg
GTX100253_39568_20160106_IFA_19011506_731.jpg
GTX100253_39568_WB_R_19011506_838.jpg
GTX100253_WB_19011506_692.jpg
GTX100253_39568_IHC_19011506_769.jpg

Cat No. GTX100253

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

HeLa , HepG2 , NIH-3T3 , JC , PC-12 , mouse brain , rat brain

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Cortactin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cortactin,Ems1,Cttn

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection , lamellipodium , ruffle

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100253_39568_20170309_WB_KO_watermark_19011506_807.jpg

GTX100253 WB Image

KO/KD

Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (GTX100253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX100253_39568_WB_M_19011506_162.jpg

GTX100253 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
7.5% SDS PAGE
GTX100253 diluted at 1:1000

GTX100253_39568_20170518_WB_M_R_19011506_210.jpg

GTX100253 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1], N-term (GTX100253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 65 kDa, 70 kDa , and 74 kDa. It is possibly due to alternative splicing.
Reference:PMID: 27903975

GTX100253_39568_20160106_IFA_19011506_731.jpg

GTX100253 ICC/IF Image

Cortactin antibody [N1], N-term detects Cortactin protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cortactin protein stained by Cortactin antibody [N1], N-term (GTX100253) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX100253_39568_WB_R_19011506_838.jpg

GTX100253 WB Image

Sample (30 ug of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX100253 diluted at 1:3000

GTX100253_WB_19011506_692.jpg

GTX100253 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX100253 diluted at 1:1000

GTX100253_39568_IHC_19011506_769.jpg

GTX100253 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cortactin(GTX100253) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169