Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cortactin antibody [N1C1] detects Cortactin protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cortactin antibody [N1C1] (GTX113680) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cortactin(GTX113680) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000.

Immunofluorescence analysis of methanol-fixed HeLa, using Cortactin(GTX113680) antibody at 1:200 dilution.