APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
PC-12 , Rat2 , Neuro 2A , C8D30 , NIH-3T3 , C2C12 , mouse brain , rat brain , HeLa
Predict Reactivity
Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.28 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Cortactin. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10618119
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cortactin , EMS1
Cellular Localization
Cytoplasm , cytoskeleton , Cell projection , lamellipodium , ruffle
Background
This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Cortactin antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113680](/templates/default/js/lightbox/images/loading.gif "Anti-Cortactin antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113680")
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GTX113680 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using Cortactin(GTX113680) antibody at 1:200 dilution.
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GTX113680 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000.
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GTX113680 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Cortactin(GTX113680) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113680 IHC-P Image
Cortactin antibody [N1C1] detects Cortactin protein at cytoplasm in mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Cortactin antibody [N1C1] (GTX113680) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113680 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Multiple bands were observed 65 kDa, 70 kDa , and 74 kDa. It is possibly due to alternative splicing.
Reference:PMID: 27903975
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GTX113680 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000.
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GTX113680 WB Image
Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody [N1C1] (GTX113680) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for Cortactin antibody [N1C1] (GTX113680). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cortactin antibody [N1C1] (GTX113680). Be the first to share your experience with this product.
![Anti-Cortactin antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113680](/upload/website/prouct_img/small/GTX113680/GTX113680_IFA_w_23060501_853.webp "Anti-Cortactin antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113680")
![Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680](/upload/website/prouct_img/small/GTX113680/GTX113680_40219_20160121_WB_M_w_23060501_422.webp "Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680")
![Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680](/upload/website/prouct_img/small/GTX113680/GTX113680_40219_IHC_w_23060501_453.webp "Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680")
![Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680](/upload/website/prouct_img/small/GTX113680/GTX113680_40219_20150612_IHC_M_w_23060501_349.webp "Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680")
![Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680](/upload/website/prouct_img/small/GTX113680/GTX113680_40219_20170518_WB_M_R_w_23060501_732.webp "Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680")
![Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680](/upload/website/prouct_img/small/GTX113680/GTX113680_40219_20160114_WB_R_w_23060501_203.webp "Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680")
![Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680](/upload/website/prouct_img/small/GTX113680/GTX113680_40219_20170309_WB_KO_watermark_w_23060501_416.webp "Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680")
![Anti-Cortactin antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113680](/upload/website/prouct_img/normal/GTX113680/GTX113680_IFA_w_23060501_853.webp "Anti-Cortactin antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113680")
![Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680](/upload/website/prouct_img/normal/GTX113680/GTX113680_40219_20160121_WB_M_w_23060501_422.webp "Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680")
![Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680](/upload/website/prouct_img/normal/GTX113680/GTX113680_40219_IHC_w_23060501_453.webp "Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680")
![Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680](/upload/website/prouct_img/normal/GTX113680/GTX113680_40219_20150612_IHC_M_w_23060501_349.webp "Anti-Cortactin antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113680")
![Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680](/upload/website/prouct_img/normal/GTX113680/GTX113680_40219_20170518_WB_M_R_w_23060501_732.webp "Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680")
![Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680](/upload/website/prouct_img/normal/GTX113680/GTX113680_40219_20160114_WB_R_w_23060501_203.webp "Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680")
![Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680](/upload/website/prouct_img/normal/GTX113680/GTX113680_40219_20170309_WB_KO_watermark_w_23060501_416.webp "Anti-Cortactin antibody [N1C1] used in Western Blot (WB). GTX113680")