Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody (GTX113681) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (5 ug of whole cell lysate)
A: 293T
B: HeLa
7.5% SDS PAGE
GTX113681 diluted at 1:10000