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Cortactin antibody

KO/KD
Anti-Cortactin antibody used in Western Blot (WB). GTX113681
Anti-Cortactin antibody used in Western Blot (WB). GTX113681

Cat. No. GTX113681

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Cortactin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:50000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Positive Control

293T , HeLa

Predict Reactivity

Mouse, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cortactin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177868

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cortactin , EMS1

Cellular Localization

Cytoplasm , cytoskeleton , Cell projection , lamellipodium , ruffle

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cortactin antibody used in Western Blot (WB). GTX113681

GTX113681 WB Image

Sample (5 ug of whole cell lysate)
A: 293T
B: HeLa
7.5% SDS PAGE
GTX113681 diluted at 1:10000

Anti-Cortactin antibody used in Western Blot (WB). GTX113681

GTX113681 WB Image

KO/KD

Wild-type (WT) and Cortactin knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Cortactin antibody (GTX113681) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Kruse K et al. Am J Physiol Lung Cell Mol Physiol 2019; Analysis of biological networks in the endothelium with biomimetic microsystem platform.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169