ICC/IF analysis of NIH-3T3 cells treated with 1.6 mM hydrogen peroxide for 1 h using GTX24904 Cortactin (phospho Tyr421) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

WB analysis of membrane extracts (30 μg lysate) of HeLa (Lane 1), and HeLa treated with Hydrogen peroxide (Lane 2) using GTX24904 Cortactin (phospho Tyr421) antibody.
Dilution : 1:250