Cortactin (phospho Tyr421) antibody

Cat. No. GTX24904
Cat. No.  GTX24904
Orthogonal
Anti-Cortactin (phospho Tyr421) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24904
GTX24904 ICC/IF Image
Orthogonal

ICC/IF analysis of NIH-3T3 cells treated with 1.6 mM hydrogen peroxide for 1 h using GTX24904 Cortactin (phospho Tyr421) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes

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Anti-Cortactin (phospho Tyr421) antibody used in Western Blot (WB). GTX24904
GTX24904 WB Image
Orthogonal

WB analysis of membrane extracts (30 μg lysate) of HeLa (Lane 1), and HeLa treated with Hydrogen peroxide (Lane 2) using GTX24904 Cortactin (phospho Tyr421) antibody.
Dilution : 1:250

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Anti-Cortactin (phospho Tyr421) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24904
Anti-Cortactin (phospho Tyr421) antibody used in Western Blot (WB). GTX24904
This Cortactin (phospho Tyr421) antibody (GTX24904) is a rabbit polyclonal antibody validated for detection of Cortactin (phospho Tyr421) by WB, ICC/IF, and FCM. Reactivity has been shown in Human and Mouse.
  • Host
    Rabbit
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Applications
    WB ICC/IF FCM
  • Reactivity
    Human, Mouse
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