GeneTex
United States (US)

Cortactin (phospho Tyr466) antibody

GTX32326_20190131_ICCIF_19013101_232.jpg
GTX32326_20190131_IHC-P_19013101_598.jpg
GTX32326_20190131_WB_19013101_954.jpg

Cat No. GTX32326

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

62 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Cortactin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cortactin,Ems1,Cttn

Cellular Localization

Cell cortex,Cell junction,Cell membrane,Cell projection,Clathrin-coated pit,Cytoplasm,Cytoskeleton,Dendrite,Dendritic spine,Focal adhesion,Lamellipodium,Membrane,Podosome,Ruffle

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES
GTX32326_20190131_ICCIF_19013101_232.jpg

GTX32326 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32326 Cortactin (phospho Tyr466) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32326_20190131_IHC-P_19013101_598.jpg

GTX32326 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32326 Cortactin (phospho Tyr466) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32326_20190131_WB_19013101_954.jpg

GTX32326 WB Image

WB analysis of UV-treated HeLa (A), H2O2-treated NIH3T3 (B) whole cell lysates using GTX32326 Cortactin (phospho Tyr466) antibody.

Package List Price ($)
$ 319