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Cortactin (phospho Tyr466) antibody

Anti-Cortactin (phospho Tyr466) antibody used in Western Blot (WB). GTX50295
Anti-Cortactin (phospho Tyr466) antibody used in Western Blot (WB). GTX50295

Cat. No. GTX50295

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all Cortactin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine 466 (P-V-Y(p)-E-T) derived from human Cortactin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cortactin , EMS1

Cellular Localization

Cytoplasm, cytoskeleton,Cell projection, dendrite,Cell membrane,Cell junction

Background

This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-Cortactin (phospho Tyr466) antibody used in Western Blot (WB). GTX50295

GTX50295 WB Image

WB analysis of extracts from Jurkat cells untreated or treated with H2O2 using GTX50295 Cortactin (phospho Tyr466) antibody.

Anti-Cortactin (phospho Tyr466) antibody used in Western Blot (WB). GTX50295

GTX50295 WB Image

WB analysis of extracts from JK cells treated with H2O2 or calf intestinal phosphatase (CIP) using GTX50295 Cortactin (phospho Tyr466) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399