APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
1:100-1:1000 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rabbit, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.05 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Coxsackie Adenovirus Receptor. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11175493
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
CXADR Ig-like cell adhesion molecule , CAR , CAR4/6 , HCAR
Cellular Localization
Isoform 1: Cell membrane , Cell junction , tight junction , adherens junction , Basolateral cell membrane , Isoform 2: Cell membrane , Basolateral cell membrane , Isoform 3: Secreted , Isoform 4: Secreted , Isoform 5: Secreted
Background
The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX118382 ICC/IF Image
Coxsackie Adenovirus Receptor antibody detects Coxsackie Adenovirus Receptor protein at cell membrane by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Coxsackie Adenovirus Receptor stained by Coxsackie Adenovirus Receptor antibody (GTX118382) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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GTX118382 IHC-P Image
Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using CAR(GTX118382) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX118382 IP Image
Immunoprecipitation of CAR protein from HeLa whole cell extracts using 5 μg of CAR antibody (GTX118382). Western blot analysis was performed using CAR antibody (GTX118382). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX118382 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coxsackie Adenovirus Receptor antibody (GTX118382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
REVIEW
Coxsackie Adenovirus Receptor antibody
Cat. No. GTX118382
Rating
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( Average 4 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 27-Aug-2018
Application Tested :
WB
Sample Species :
Hu
Amount used :
20 μg
Blocking :
10 % milk in PBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr