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Coxsackie Adenovirus Receptor antibody

Anti-Coxsackie Adenovirus Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118382
Anti-Coxsackie Adenovirus Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX118382
Anti-Coxsackie Adenovirus Receptor antibody used in Immunoprecipitation (IP). GTX118382
Anti-Coxsackie Adenovirus Receptor antibody used in Western Blot (WB). GTX118382

Cat. No. GTX118382

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Predict Reactivity

Mouse, Rabbit, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Coxsackie Adenovirus Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175493

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CXADR Ig-like cell adhesion molecule , CAR , CAR4/6 , HCAR

Cellular Localization

Isoform 1: Cell membrane , Cell junction , tight junction , adherens junction , Basolateral cell membrane , Isoform 2: Cell membrane , Basolateral cell membrane , Isoform 3: Secreted , Isoform 4: Secreted , Isoform 5: Secreted

Background

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Coxsackie Adenovirus Receptor antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118382

GTX118382 ICC/IF Image

Coxsackie Adenovirus Receptor antibody detects Coxsackie Adenovirus Receptor protein at cell membrane by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Coxsackie Adenovirus Receptor stained by Coxsackie Adenovirus Receptor antibody (GTX118382) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Coxsackie Adenovirus Receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX118382

GTX118382 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using CAR(GTX118382) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Coxsackie Adenovirus Receptor antibody used in Immunoprecipitation (IP). GTX118382

GTX118382 IP Image

Immunoprecipitation of CAR protein from HeLa whole cell extracts using 5 μg of CAR antibody (GTX118382).
Western blot analysis was performed using CAR antibody (GTX118382).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Coxsackie Adenovirus Receptor antibody used in Western Blot (WB). GTX118382

GTX118382 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coxsackie Adenovirus Receptor antibody (GTX118382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

Coxsackie Adenovirus Receptor antibody Cat. No. GTX118382

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 27-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 20 μg
Blocking : 10 % milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169