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Cpn10 antibody [M1.2]

Anti-Cpn10 antibody [M1.2] used in Western Blot (WB). GTX13527

Cat. No. GTX13527

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 M1.2

Isotype

 IgG2a

Application

 WB, IP, EIA

Reactivity

 Human
Package
50 μg ($399)
See all Cpn10 products

APPLICATION

Application Note

EIA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.25 μg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 9.9 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

11 kDa. ( Note )

Product Note

This antibody will not react with the human or mouse cpn10 homolog nor with E. coli GroES.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1mM PMSF

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A 16 residue synthetic MAP peptide KKDDKGQQLPFEVQVG based on C. trachomatis cpn10 (residues 54-69).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family E (Hsp10) member 1 , CPN10 , EPF , GROES , HSP10

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-Cpn10 antibody [M1.2] used in Western Blot (WB). GTX13527

GTX13527 WB Image

Western blot analysis of chlamydia-infected human HeLa cell lysate, probed with GTX13527

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399