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Cpn10 antibody

Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
Anti-Cpn10 antibody used in Western Blot (WB). GTX115575
Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575
Anti-Cpn10 antibody used in IHC (Paraffin sections) (IHC-P). GTX115575

Cat. No. GTX115575

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Cpn10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cpn10 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619841

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family E (Hsp10) member 1 , CPN10 , EPF , GROES , HSP10

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575

GTX115575 ICC/IF Image

Cpn10 antibody detects Cpn10 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cpn10 protein stained by Cpn10 antibody (GTX115575) diluted at 1:100.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-Cpn10 antibody used in Western Blot (WB). GTX115575

GTX115575 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX115575 diluted at 1:1000

Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575

GTX115575 ICC/IF Image

Cpn10 antibody detects Cpn10 protein at cytoplasm by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cpn10 protein stained by Cpn10 antibody (GTX115575) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-Cpn10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115575

GTX115575 ICC/IF Image

Cpn10 antibody detects Cpn10 protein at mitochondria by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 15 min.
Green: Cpn10 protein stained by Cpn10 antibody (GTX115575) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Cpn10 antibody used in IHC (Paraffin sections) (IHC-P). GTX115575

GTX115575 IHC-P Image

Cpn10 antibody detects Cpn10 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded lung carcinoma.
Cpn10 antibody (GTX115575) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Rappa F et al. Anticancer Res 2014; 34 (8) : 823137 Comparative analysis of hsp10 and hsp90 expression in healthy mucosa and adenocarcinoma of the large bowel.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

Cpn10 antibody Cat. No. GTX115575

Rating
( Average 3 based on 1 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Anti-Cpn10 antibody used in Western Blot (WB). GTX115575
Date : Anonymous submitted on 24-Sep-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HK2 cells
Amount used : 40μg
Blocking : 3% BSA, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169