GeneTex
  • Country / Location Selection

United States (US)

Cre recombinase antibody [GT10212]

Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479
Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479
Anti-Cre recombinase antibody [GT10212] used in Immunoprecipitation (IP). GTX629479

Cat No. GTX629479

Host Mouse
Clonality Monoclonal
Clone Name GT10212
Isotype IgG2a
Application WB, IP
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Cre-tagged protein expressing HeLa , IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cre antibody

Database

Research Area

DATA IMAGES
Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479

GTX629479 WB Image

Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis.
A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
12.5% SDS-PAGE
Cre recombinase antibody [GT10212] (GTX629479) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479

GTX629479 WB Image

Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis.
A. 30 µg HeLa whole cell lysate/extract
B. 30 µg whole cell lysate/extract of Cre-tagged protein expressing HeLa cells
7.5% SDS-PAGE
Cre recombinase antibody [GT10212] (GTX629479) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cre recombinase antibody [GT10212] used in Immunoprecipitation (IP). GTX629479

GTX629479 IP Image

Cre recombinase antibody immunoprecipitates Cre recombinase protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 μg of Cre recombinase antibody (GTX629479) 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody (GTX629479) diluted at 1 : 5000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

REFERENCE
There are currently no references for Cre recombinase antibody [GT10212] (GTX629479).
Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cre recombinase antibody [GT10212] (GTX629479).
Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169