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Cre recombinase antibody [GT10212]

Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479
Anti-Cre recombinase antibody [GT10212] used in Immunoprecipitation (IP). GTX629479
Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479

Cat. No. GTX629479

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT10212

Isotype

 IgG2a

Application

 WB, IP
Package
100 μl ($399),
25 μl ($169)
See all Cre recombinase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Cre-tagged protein expressing HeLa cell

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888117

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479

GTX629479 WB Image

Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis.
A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
12.5% SDS-PAGE
Cre recombinase antibody [GT10212] (GTX629479) dilution: 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cre recombinase antibody [GT10212] used in Immunoprecipitation (IP). GTX629479

GTX629479 IP Image

Cre recombinase antibody immunoprecipitates Cre recombinase protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 μg of Cre recombinase antibody (GTX629479) 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody (GTX629479) diluted at 1 : 5000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-Cre recombinase antibody [GT10212] used in Western Blot (WB). GTX629479

GTX629479 WB Image

Cre recombinase antibody [GT10212] detects Cre recombinase protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Cre-tagged protein expressing HeLa cells
7.5% SDS-PAGE
Cre recombinase antibody [GT10212] (GTX629479) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Wang Z et al. Vaccines (Basel) 2021; 9 (7) In Vivo Production of HN Protein Increases the Protection Rates of a Minicircle DNA Vaccine against Genotype VII Newcastle Disease Virus.
Jiang Y et al. Appl Environ Microbiol 2019; (Epub) A novel Cre recombinase-mediated in vivo minicircle DNA (CRIM) vaccine provides partial protection against Newcastle disease virus.
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169