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Cre recombinase antibody

Anti-Cre recombinase antibody used in Western Blot (WB). GTX127270
Anti-Cre recombinase antibody used in Western Blot (WB). GTX127270

Cat No. GTX127270

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T transfected recombinant cre protein , IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Cre recombinase antibody used in Western Blot (WB). GTX127270

GTX127270 WB Image

Cre antibody detects Cre protein by western blot analysis.
A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
12.5 % SDS-PAGE
Cre antibody (GTX127270) dilution: 1:10000

Anti-Cre recombinase antibody used in Western Blot (WB). GTX127270

GTX127270 WB Image

Cre recombinase antibody detects Cre recombinase protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Cre-ERT2-transfected 293T cells (Cre-ERT2 fusion protein size: 72kDa)
7.5 % SDS-PAGE
Cre recombinase antibody (GTX127270) dilution: 1:10000

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169