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Cre recombinase antibody

Anti-Cre recombinase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127270
Anti-Cre recombinase antibody used in Western Blot (WB). GTX127270
Anti-Cre recombinase antibody used in Western Blot (WB). GTX127270

Cat. No. GTX127270

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF
Package
100 μl ($399),
25 μl ($169)
See all Cre recombinase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

cre protein transfected 293T , Cre recombinant proteins

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.27 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171781

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Cre recombinase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX127270

GTX127270 ICC/IF Image

Cre recombinase antibody detects Cre recombinase protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cre recombinase stained by Cre recombinase antibody (GTX127270) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Cre recombinase antibody used in Western Blot (WB). GTX127270

GTX127270 WB Image

Cre antibody detects Cre protein by western blot analysis.
A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
12.5 % SDS-PAGE
Cre antibody (GTX127270) dilution: 1:10000

Anti-Cre recombinase antibody used in Western Blot (WB). GTX127270

GTX127270 WB Image

Cre recombinase antibody detects Cre recombinase protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Cre-ERT2-transfected 293T cells (Cre-ERT2 fusion protein size: 72kDa)
7.5 % SDS-PAGE
Cre recombinase antibody (GTX127270) dilution: 1:10000

REFERENCE

Application Reference

Lee KY et al. Hum Mol Genet 2022; 31 (18) : 3144-3160 Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy.
Iershov A et al. Nat Commun 2019; 10 (1) : 1566 The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα.
Application : WB
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169