GeneTex
United States (US)

Cre recombinase antibody

GTX127271_40947_WB_Recombinant-protein_18121410_784.jpg
GTX127271_40947_IP_18121410_739.jpg
GTX127271_40947_WB_B_18121410_328.jpg

Cat No. GTX127271

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T transfected recombinant cre protein , IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein conjugated synthetic peptide encompassing a sequence within the center region of Cre recombinase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cre antibody

Database

Research Area

DATA IMAGES
GTX127271_40947_WB_Recombinant-protein_18121410_784.jpg

GTX127271 WB Image

Cre recombinase antibody detects Cre recombinase protein by western blot analysis.
A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
12.5% SDS-PAGE
Cre recombinase antibody (GTX127271) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX127271_40947_IP_18121410_739.jpg

GTX127271 IP Image

Cre recombinase antibody immunoprecipitates Cre recombinase protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 μg of Cre recombinase antibody (GTX127271) 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody (GTX127271) diluted at 1 : 5000. EasyBlot anti-mouse IgG (HRP) (GTX221666-01) was used as a secondary reagent.

GTX127271_40947_WB_B_18121410_328.jpg

GTX127271 WB Image

Cre recombinase antibody detects Cre recombinase protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of Cre-ERT2-transfected 293T cells (Cre-ERT2 fusion protein size: 72kDa)
7.5% SDS-PAGE
Cre recombinase antibody (GTX127271) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169