Cre recombinase antibody

Cre recombinase antibody detects Cre recombinase protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Cre-ERT2-transfected 293T cells (Cre-ERT2 fusion protein size: 72kDa)
7.5% SDS-PAGE
Cre recombinase antibody (GTX127271) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cre recombinase antibody detects Cre recombinase protein by western blot analysis.
A. IPTG-induced cell lysate from E. coli expressing Cre recombinant proteins
12.5% SDS-PAGE
Cre recombinase antibody (GTX127271) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cre recombinase antibody immunoprecipitates Cre recombinase protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of Cre recombinase protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Cre recombinase protein by 2.6 μg of Cre recombinase antibody (GTX127271) 7.5% SDS-PAGE The immunoprecipitated Cre recombinase protein was detected by Cre recombinase antibody (GTX127271) diluted at 1 : 5000. EasyBlot anti-mouse IgG (HRP) (GTX221666-01) was used as a secondary reagent.