Creatine kinase MT antibody

Cat. No. GTX103627

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, IHC-Wm
Reactivity	Human, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all Creatine kinase MT products

Datasheet
Document

View product citations for antibody GTX103627 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000
IHC-Wm	Assay dependent

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

H1299 , HeLaS3

Predict Reactivity

Mouse, Rat, Rabbit, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase MT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949988

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

creatine kinase, mitochondrial 1B , CKMT , CKMT1 , UMTCK

Cellular Localization

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side

Background

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq]

Database

Gene ID: 1159 CKMT1B
UniProt: P12532 CKMT1B

Research Area

DATA IMAGES

GTX103627 IHC-Wm Image

Creatine kinase MT antibody detects Creatine kinase MT protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Creatine kinase MT antibody (GTX103627) dilution: 1:100.

GTX103627 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
10% SDS PAGE
GTX103627 diluted at 1:1000

GTX103627 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Creatine kinase 1B(GTX103627) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for Creatine kinase MT antibody (GTX103627). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for Creatine kinase MT antibody (GTX103627). Be the first to share your experience with this product.

Submit a Review

SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty