GeneTex
  • Country / Location Selection

United States (US)

Creatine kinase (brain) antibody [N1C1]

Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101759
Anti-Creatine kinase (brain) antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101759

Cat No. GTX101759

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , H1299 , HeLaS3 , HepG2 , NIH-3T3

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase (brain). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

creatine kinase B , B-CK , BCK , CKBB , CPK-B , HEL-211 , HEL-S-29

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759

GTX101759 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (brain) antibody [N1C1] used in Western Blot (WB). GTX101759

GTX101759 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (brain) antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101759

GTX101759 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Creatine kinase (brain) antibody(GTX101759) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Creatine kinase (brain) antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101759

GTX101759 ICC/IF Image

Creatine kinase (brain) antibody [N1C1] detects Creatine kinase (brain) protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Creatine kinase (brain) protein stained by Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW
There are currently no reviews for Creatine kinase (brain) antibody [N1C1] (GTX101759). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169