Creatine kinase (brain) antibody [N1C1]

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Creatine kinase (brain) antibody(GTX101759) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Creatine kinase (brain) antibody [N1C1] detects Creatine kinase (brain) protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Creatine kinase (brain) protein stained by Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Creatine kinase (brain) antibody [N1C1] detects Creatine kinase (brain) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Creatine kinase (brain) stained by Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Creatine kinase (brain) antibody [N1C1] detects Creatine kinase (brain) protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Creatine kinase (brain) stained by Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Creatine kinase (brain) antibody [N1C1] (GTX101759) at 1:300 dilution.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX101759 diluted at 1:1000