GeneTex
United States (US)

Creatine kinase (brain) antibody [N1C1]

GTX101759_WB_19011506_875.jpg
GTX101759_39577_IHC_19011506_648.jpg
GTX101759_WB_M_19011506_592.jpg
GTX101759_39577_20150410_IFA_19011506_563.jpg

Cat No. GTX101759

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , H1299 , HeLaS3 , HepG2 , NIH-3T3

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase (brain). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Creatine Kinase B,B-Ck,Bck,Ckbb,Cpk-B,Hel-211,Hel-S-29,Ckb

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101759_WB_19011506_875.jpg

GTX101759 WB Image

Sample (30 µg whole cell lysate)
A:H1299
10% SDS PAGE
GTX101759 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101759_39577_IHC_19011506_648.jpg

GTX101759 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Creatine kinase (brain) antibody(GTX101759) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101759_WB_M_19011506_592.jpg

GTX101759 WB Image

Sample (30 µg of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
Creatine Kinase BB antibody
GTX101759 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101759_39577_20150410_IFA_19011506_563.jpg

GTX101759 ICC/IF Image

Creatine kinase (brain) antibody [N1C1] detects Creatine kinase (brain) protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Creatine kinase (brain) protein stained by Creatine kinase (brain) antibody [N1C1] (GTX101759) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169