Creatine kinase (brain) antibody [N3C3]

Name: Creatine kinase (brain) antibody [N3C3]
Brand: GeneTex
SKU: GTX101760
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Creatine kinase (brain) antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101760

Anti-Creatine kinase (brain) antibody [N3C3] used in Western Blot (WB). GTX101760

Anti-Creatine kinase (brain) antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101760

Cat. No. GTX101760

Reference 3

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat, Zebrafish
Package	100 μl ($399), 25 μl ($169)

See all Creatine kinase (brain) products

View product citations for antibody GTX101760 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

Mouse brain , H1299 , HeLaS3 , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase (brain). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949985

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

creatine kinase B , B-CK , BCK , CKBB , CPK-B , HEL-211 , HEL-S-29

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]

Database

Gene ID: 1152 CKB
UniProt: P12277 CKB

Research Area

DATA IMAGES

GTX101760 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB(GTX101760) antibody at 1:100 dilution.

GTX101760 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101760 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101760 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Creatine kinase (brain)(GTX101760) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101760 WB Image

Creatine kinase (brain) antibody detects Creatine kinase protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and blotted with Creatine kinase (brain) antibody (GTX101760) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101760 WB Image

Sample(30 μg of whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX101760 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101760 IHC-P Image

Creatine kinase (brain) antibody [N3C3] detects Creatine kinase (brain) protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Creatine kinase (brain) antibody [N3C3] (GTX101760) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101760 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Creatine kinase (brain) antibody [N3C3] (GTX101760) at 1:300 dilution.

GTX101760 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX101760 diluted at 1:1000

REFERENCE

Application Reference

Huang PY et al. Viruses 2020; 12 (9) Screening for the Proteins That Can Interact with Grouper Nervous Necrosis Virus Capsid Protein.

Application : WB

Borgo C et al. Sci Rep 2017; 7 : 42409 Generation and quantitative proteomics analysis of CK2α/α'^(-/-) cells.

Application : WB

Reactivity : Mouse

Buchkremer S et al. JND 2016; 3 (2) : 169-181 Sil1-Mutant Mice Elucidate Chaperone Function in Neurological Disorders.

Application : IHC-P

Reactivity : Mouse

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