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Creatine kinase (brain) antibody [N3C3]

Anti-Creatine kinase (brain) antibody [N3C3] used in Western Blot (WB). GTX101760
Anti-Creatine kinase (brain) antibody [N3C3] used in Western Blot (WB). GTX101760
Anti-Creatine kinase (brain) antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101760
Anti-Creatine kinase (brain) antibody [N3C3] used in Western Blot (WB). GTX101760
Anti-Creatine kinase (brain) antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101760
Anti-Creatine kinase (brain) antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101760

Cat No. GTX101760

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

H1299 , HeLaS3 , mouse brain , rat brain

Predict Reactivity

Rabbit, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase (brain). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

creatine kinase B , B-CK , BCK , CKBB , CPK-B , HEL-211 , HEL-S-29

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Creatine kinase (brain) antibody [N3C3] used in Western Blot (WB). GTX101760

GTX101760 WB Image

Sample(30 µg of whole cell lysate)
A:H1299
B:HeLa S3(GTX14654)
12% SDS PAGE
GTX101760 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (brain) antibody [N3C3] used in Western Blot (WB). GTX101760

GTX101760 WB Image

Creatine kinase (brain) antibody detects Creatine kinase protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and blotted with Creatine kinase (brain) antibody (GTX101760) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (brain) antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101760

GTX101760 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Creatine kinase (brain)(GTX101760) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Creatine kinase (brain) antibody [N3C3] used in Western Blot (WB). GTX101760

GTX101760 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101760 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (brain) antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101760

GTX101760 IHC-P Image

Creatine kinase (brain) antibody [N3C3] detects Creatine kinase (brain) protein at cytoplasm on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Creatine kinase (brain) antibody [N3C3] (GTX101760) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Creatine kinase (brain) antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101760

GTX101760 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Creatine Kinase BB(GTX101760) antibody at 1:100 dilution.

REFERENCE
REVIEW

Creatine kinase (brain) antibody [N3C3] Cat No. GTX101760

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 07-Apr-2014
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse brain tissue
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 25°C, 1Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169