APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HeLa
Predict Reactivity
Mouse, Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.71 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human Creatine kinase (muscle). The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10731275
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
creatine kinase, M-type , CKMM , CPK-M , M-CK
Cellular Localization
Cytoplasm
Background
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-Creatine kinase (muscle) antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103792](/templates/default/js/lightbox/images/loading.gif "Anti-Creatine kinase (muscle) antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103792")
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GTX103792 IHC-P Image
Creatine kinase (muscle) detects Creatine kinase (muscle) protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat muscle.Creatine kinase (muscle) stained by Creatine kinase (muscle) antibody (GTX103792) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX103792 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX103792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103792 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX103792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103792 WB Image
Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX103792 diluted at 1:1000
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REFERENCE
There are currently no references for Creatine kinase (muscle) antibody [N1C3] (GTX103792). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Creatine kinase (muscle) antibody [N1C3] (GTX103792). Be the first to share your experience with this product.
![Anti-Creatine kinase (muscle) antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103792](/upload/website/prouct_img/small/GTX103792/GTX103792_40478_20190802_IHC-P_C_22111423_304.webp "Anti-Creatine kinase (muscle) antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103792")
![Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792](/upload/website/prouct_img/small/GTX103792/GTX103792_40478_20190201_WB_D_22111423_380.webp "Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792")
![Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792](/upload/website/prouct_img/small/GTX103792/GTX103792_40478_20190322_WB_C_22111423_770.webp "Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792")
![Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792](/upload/website/prouct_img/small/GTX103792/GTX103792_40478_WB_w_23060120_302.webp "Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792")
![Anti-Creatine kinase (muscle) antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103792](/upload/website/prouct_img/normal/GTX103792/GTX103792_40478_20190802_IHC-P_C_22111423_304.webp "Anti-Creatine kinase (muscle) antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103792")
![Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792](/upload/website/prouct_img/normal/GTX103792/GTX103792_40478_20190201_WB_D_22111423_380.webp "Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792")
![Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792](/upload/website/prouct_img/normal/GTX103792/GTX103792_40478_20190322_WB_C_22111423_770.webp "Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792")
![Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792](/upload/website/prouct_img/normal/GTX103792/GTX103792_40478_WB_w_23060120_302.webp "Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792")