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Creatine kinase (muscle) antibody [N1C3]

Anti-Creatine kinase (muscle) antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103792
Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792
Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792
Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792

Cat. No. GTX103792

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Cat, Dog
Package
100 μl ($399),
25 μl ($169)
See all Creatine kinase (muscle) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase (muscle). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731275

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

creatine kinase, M-type , CKMM , CPK-M , M-CK

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Creatine kinase (muscle) antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX103792

GTX103792 IHC-P Image

Creatine kinase (muscle) detects Creatine kinase (muscle) protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat muscle.
Creatine kinase (muscle) stained by Creatine kinase (muscle) antibody (GTX103792) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792

GTX103792 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX103792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792

GTX103792 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX103792) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (muscle) antibody [N1C3] used in Western Blot (WB). GTX103792

GTX103792 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX103792 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169