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Creatine kinase (muscle) antibody [N2C3]

Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969

Cat No. GTX108969

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

293T , A431 , HeLaS3

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase (muscle). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

creatine kinase, M-type,CKMM,CPK-M,M-CK

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969

GTX108969 IHC-P Image

Creatine kinase (muscle) antibody [N2C3] detects Creatine kinase (muscle) protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Creatine kinase (muscle) antibody [N2C3] (GTX108969) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969

GTX108969 WB Image

Sample (30 ug of whole cell lysate)
A: Hela S3
10% SDS PAGE
GTX108969 diluted at 1:1000

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969

GTX108969 WB Image

Creatine kinase (muscle) antibody detects Creatine kinase (muscle) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX108969) diluted by 1:1000.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169