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Creatine kinase (muscle) antibody [N2C3]

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Immunohistochemistry (IHC). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969
Anti-Creatine kinase (muscle) antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108969

Cat. No. GTX108969

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IHC

Reactivity

 Human, Mouse, Zebrafish, Dog
Package
100 μl ($399),
25 μl ($169)
See all Creatine kinase (muscle) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLaS3 , 293T , A431

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Creatine kinase (muscle). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949986

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

creatine kinase, M-type , CKMM , CPK-M , M-CK

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969

GTX108969 WB Image

Creatine kinase (muscle) antibody detects Creatine kinase (muscle) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX108969) diluted by 1:1000.

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969

GTX108969 WB Image

Sample (30 ug of whole cell lysate)
A: Hela S3
10% SDS PAGE
GTX108969 diluted at 1:1000

Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969

GTX108969 IHC-P Image

Creatine kinase (muscle) antibody [N2C3] detects Creatine kinase (muscle) protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Creatine kinase (muscle) antibody [N2C3] (GTX108969) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Creatine kinase (muscle) antibody [N2C3] used in Western Blot (WB). GTX108969

GTX108969 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Creatine kinase (muscle) antibody (GTX108969) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Creatine kinase (muscle) antibody [N2C3] used in Immunohistochemistry (IHC). GTX108969

GTX108969 IHC Image

Immunohistochemical analysis of agarose-embedded zebrafish embryo tail section, using Creatine kinase (muscle) antibody [N2C3] (GTX108969) at 1:200 dilution.

Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Whole mount) (IHC-Wm). GTX108969

GTX108969 IHC-Wm Image

Creatine kinase (muscle) antibody [N2C3] detects Ckma protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Creatine kinase (muscle) antibody [N2C3] (GTX108969) dilution: 1:100.
Anti-Creatine kinase (muscle) antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX108969

GTX108969 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Creatine kinase (muscle) antibody [N2C3] (GTX108969) at 1:300 dilution.

Anti-Creatine kinase (muscle) antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108969

GTX108969 ICC/IF Image

Creatine kinase (muscle) detects Creatine kinase (muscle) protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Creatine kinase (muscle) stained by Creatine kinase (muscle) antibody (GTX108969) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Wakao J et al. Biochem Biophys Res Commun 2017; (Epub) Efficient direct conversion of human fibroblasts into myogenic lineage induced by co-transduction with MYCL and MYOD1.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169